A new beta-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Miklós Takó, Adél Tóth, László G Nagy, Judit Krisch, Csaba Vágvölgyi, Tamás Papp

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14 Citations (Scopus)

Abstract

In this study, a beta-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identified; the two domains are connected with a 84-amino-acids linker. The catalytic region showed an extensive sequence homology with other fungal beta-glucosidases classified as family 3 glycoside hydrolases. The isolated Rhizomucor gene was expressed in the related fungus Mucor circinelloides. Transformant Mucor strains maintained the introduced plasmid in an autoreplicative manner and showed significantly higher cellobiase activity than the recipient strain.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalAntonie Van Leeuwenhoek
Volume97
Issue number1
DOIs
Publication statusPublished - Jan 2010

Keywords

  • Catalytic Domain
  • Cloning, Molecular
  • DNA, Fungal
  • Gene Expression
  • Genetic Vectors
  • Introns
  • Molecular Sequence Data
  • Mucor
  • Open Reading Frames
  • Plasmids
  • Rhizomucor
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • beta-Glucosidase

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    Takó, M., Tóth, A., G Nagy, L., Krisch, J., Vágvölgyi, C., & Papp, T. (2010). A new beta-glucosidase gene from the zygomycete fungus Rhizomucor miehei. Antonie Van Leeuwenhoek, 97(1), 1-10. https://doi.org/10.1007/s10482-009-9382-z