A new beta-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Miklós Takó; Adél Tóth; László G Nagy; Judit Krisch; Csaba Vágvölgyi; Tamás Papp

doi:10.1007/s10482-009-9382-z

A new beta-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Miklós Takó, Adél Tóth, László G Nagy, Judit Krisch, Csaba Vágvölgyi, Tamás Papp

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (SciVal)

Abstract

In this study, a beta-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identified; the two domains are connected with a 84-amino-acids linker. The catalytic region showed an extensive sequence homology with other fungal beta-glucosidases classified as family 3 glycoside hydrolases. The isolated Rhizomucor gene was expressed in the related fungus Mucor circinelloides. Transformant Mucor strains maintained the introduced plasmid in an autoreplicative manner and showed significantly higher cellobiase activity than the recipient strain.

Original language	English
Pages (from-to)	1-10
Number of pages	10
Journal	Antonie Van Leeuwenhoek
Volume	97
Issue number	1
DOIs	https://doi.org/10.1007/s10482-009-9382-z
Publication status	Published - Jan 2010

Keywords

Catalytic Domain
Cloning, Molecular
DNA, Fungal
Gene Expression
Genetic Vectors
Introns
Molecular Sequence Data
Mucor
Open Reading Frames
Plasmids
Rhizomucor
Sequence Analysis, DNA
Sequence Homology, Amino Acid
beta-Glucosidase

Access to Document

10.1007/s10482-009-9382-z

http://10.1007/s10482-009-9382-z

Cite this

@article{6adcf27768aa4b858902ce334f206e82,

title = "A new beta-glucosidase gene from the zygomycete fungus Rhizomucor miehei",

abstract = "In this study, a beta-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identified; the two domains are connected with a 84-amino-acids linker. The catalytic region showed an extensive sequence homology with other fungal beta-glucosidases classified as family 3 glycoside hydrolases. The isolated Rhizomucor gene was expressed in the related fungus Mucor circinelloides. Transformant Mucor strains maintained the introduced plasmid in an autoreplicative manner and showed significantly higher cellobiase activity than the recipient strain.",

keywords = "Catalytic Domain, Cloning, Molecular, DNA, Fungal, Gene Expression, Genetic Vectors, Introns, Molecular Sequence Data, Mucor, Open Reading Frames, Plasmids, Rhizomucor, Sequence Analysis, DNA, Sequence Homology, Amino Acid, beta-Glucosidase",

author = "Mikl{\'o}s Tak{\'o} and Ad{\'e}l T{\'o}th and {G Nagy}, L{\'a}szl{\'o} and Judit Krisch and Csaba V{\'a}gv{\"o}lgyi and Tam{\'a}s Papp",

year = "2010",

month = jan,

doi = "10.1007/s10482-009-9382-z",

language = "English",

volume = "97",

pages = "1--10",

journal = "Antonie Van Leeuwenhoek",

issn = "0003-6072",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "1",

}

TY - JOUR

T1 - A new beta-glucosidase gene from the zygomycete fungus Rhizomucor miehei

AU - Takó, Miklós

AU - Tóth, Adél

AU - G Nagy, László

AU - Krisch, Judit

AU - Vágvölgyi, Csaba

AU - Papp, Tamás

PY - 2010/1

Y1 - 2010/1

N2 - In this study, a beta-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identified; the two domains are connected with a 84-amino-acids linker. The catalytic region showed an extensive sequence homology with other fungal beta-glucosidases classified as family 3 glycoside hydrolases. The isolated Rhizomucor gene was expressed in the related fungus Mucor circinelloides. Transformant Mucor strains maintained the introduced plasmid in an autoreplicative manner and showed significantly higher cellobiase activity than the recipient strain.

AB - In this study, a beta-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identified; the two domains are connected with a 84-amino-acids linker. The catalytic region showed an extensive sequence homology with other fungal beta-glucosidases classified as family 3 glycoside hydrolases. The isolated Rhizomucor gene was expressed in the related fungus Mucor circinelloides. Transformant Mucor strains maintained the introduced plasmid in an autoreplicative manner and showed significantly higher cellobiase activity than the recipient strain.

KW - Catalytic Domain

KW - Cloning, Molecular

KW - DNA, Fungal

KW - Gene Expression

KW - Genetic Vectors

KW - Introns

KW - Molecular Sequence Data

KW - Mucor

KW - Open Reading Frames

KW - Plasmids

KW - Rhizomucor

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - beta-Glucosidase

UR - http://10.1007/s10482-009-9382-z

U2 - 10.1007/s10482-009-9382-z

DO - 10.1007/s10482-009-9382-z

M3 - Article

C2 - 19813101

SN - 0003-6072

VL - 97

SP - 1

EP - 10

JO - Antonie Van Leeuwenhoek

JF - Antonie Van Leeuwenhoek

IS - 1

ER -

A new beta-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this