A mitochondria-targeting fluorescent probe for the dual-emission fluorescence-enhanced detection of hydrogen sulfide and turn-on detection of hydrazine

Yu-Man Tian, Shuang-Shuang Liu, Wei Na Wu, Xiao Lei Zhao, Yuan Wang, Yun Chang Fan, Zhi Hong Xu, Tony James

Research output: Contribution to journalArticlepeer-review

6 Citations (SciVal)

Abstract

A fluorescent probe (probe 1) with selectivity and sensitivity for H2S and N2H4 has been developed. Probe 1 exhibits dual-excitation and dual-emission fluorescence response to H2S and fluorescence enhancement toward N2H4 with distinct naked-eye color changes. Moreover, probe 1 displayed low detection limits for H2S and N2H4 of 0.90 nM and 23.6 nM, respectively. The reaction mechanism of probe 1 toward H2S and N2H4 was established using 1H NMR and HR-MS spectroscopy. Additionally, probe 1 was used for monitoring and differentiating H2S and N2H4 in HeLa cells and displays good biocompatibility and low cytotoxicity.

Original languageEnglish
Article number135496
Number of pages10
JournalSensors and Actuators B: Chemical
Volume409
Early online date15 Feb 2024
DOIs
Publication statusPublished - 15 Jun 2024

Data Availability Statement

The authors confirm that the data supporting the findings of this research are available within the article and supplementary materials.

Funding

This work was supported in part by the National Natural Science Foundation of China (No. 21907023 and 21001040), the Joint Program for Fostering Talents of the National Natural Science Foundation of China and Henan Province (No. U1304202), the Science and Technology Department of Henan Province (No. 232102320092, 222300420523, and 212102210646), the Education Department of Henan Province (No. 24A150010), the Fundamental Research Funds for the Universities of Henan Province (No. NSFRF230402), the Double World-Class Project of Henan Polytechnic University (AQ20230745 and AQ20230754), and Key Program of Xuchang University (2022CXTD001). TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support.

FundersFunder number
University of Bath
Education Department of Henan Province24A150010
Key Program of Xuchang University2022CXTD001
Science and Technology Department of Henan Province212102210646, 232102320092, 222300420523
National Natural Science Foundation of China21001040, 21907023
Henan Polytechnic UniversityAQ20230745, AQ20230754
National Natural Science Foundation of China and Henan ProvinceU1304202
Fundamental Research Funds for the Universities of Henan ProvinceNSFRF230402
Henan Normal University2020ZD01

    Keywords

    • Cell imaging
    • Dual response
    • Hydrazine detection
    • Hydrogen sulfide

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics
    • Metals and Alloys
    • Instrumentation
    • Materials Chemistry
    • Surfaces, Coatings and Films
    • Electrical and Electronic Engineering

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