A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies

Robert N Wilkinson; Stone Elworthy; Philip W Ingham; Fredericus J M van Eeden

doi:10.2144/000114116

A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies

Robert N Wilkinson
, Stone Elworthy
, Philip W Ingham
, Fredericus J M van Eeden

Department of Life Sciences

Sheffield University Teaching Hospitals
University of Sheffield

Research output: Contribution to journal › Article › peer-review

56 Citations (SciVal)

Abstract

Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

Original language	English
Pages (from-to)	314-6
Number of pages	3
Journal	Biotechniques
Volume	55
Issue number	6
Early online date	3 Apr 2013
DOIs	https://doi.org/10.2144/000114116
Publication status	Published - 1 Dec 2013

Keywords

Animals
Biopsy/veterinary
Genotyping Techniques
High-Throughput Nucleotide Sequencing
Larva/genetics
Polymerase Chain Reaction/methods
Tail/pathology
Zebrafish/genetics

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10.2144/000114116

Cite this

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title = "A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies",

abstract = "Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.",

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AU - Ingham, Philip W

AU - van Eeden, Fredericus J M

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AB - Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

KW - Animals

KW - Biopsy/veterinary

KW - Genotyping Techniques

KW - High-Throughput Nucleotide Sequencing

KW - Larva/genetics

KW - Polymerase Chain Reaction/methods

KW - Tail/pathology

KW - Zebrafish/genetics

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A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies

Abstract

Keywords

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