A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies

Robert N Wilkinson, Stone Elworthy, Philip W Ingham, Fredericus J M van Eeden

Research output: Contribution to journalArticlepeer-review

44 Citations (SciVal)

Abstract

Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

Original languageEnglish
Pages (from-to)314-6
Number of pages3
JournalBiotechniques
Volume55
Issue number6
Early online date3 Apr 2013
DOIs
Publication statusPublished - 1 Dec 2013

Keywords

  • Animals
  • Biopsy/veterinary
  • Genotyping Techniques
  • High-Throughput Nucleotide Sequencing
  • Larva/genetics
  • Polymerase Chain Reaction/methods
  • Tail/pathology
  • Zebrafish/genetics

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