A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters

A A Caricasole; A Ward

doi:10.1016/0378-1119(93)90777-Z

A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters

A A Caricasole, A Ward

Department of Life Sciences

University of Oxford

Research output: Contribution to journal › Article › peer-review

Abstract

A luciferase-reporter plasmid vector has been constructed for the rapid subcloning and analysis of eukaryotic promoters. Identification of recombinants, when using this vector, is greatly facilitated by the retention of blue-white selection (alpha-complementation).

Original language	English
Pages (from-to)	139-40
Number of pages	2
Journal	Gene
Volume	124
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(93)90777-Z
Publication status	Published - 14 Feb 1993

Keywords

Animals
Cloning, Molecular
Coleoptera
Genetic Vectors
Introns
Luciferases
Plasmids
Promoter Regions, Genetic
Restriction Mapping
Simian virus 40
Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0378-1119(93)90777-Z

Cite this

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title = "A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters",

abstract = "A luciferase-reporter plasmid vector has been constructed for the rapid subcloning and analysis of eukaryotic promoters. Identification of recombinants, when using this vector, is greatly facilitated by the retention of blue-white selection (alpha-complementation).",

keywords = "Animals, Cloning, Molecular, Coleoptera, Genetic Vectors, Introns, Luciferases, Plasmids, Promoter Regions, Genetic, Restriction Mapping, Simian virus 40, Journal Article, Research Support, Non-U.S. Gov't",

author = "Caricasole, \{A A\} and A Ward",

year = "1993",

month = feb,

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doi = "10.1016/0378-1119(93)90777-Z",

language = "English",

volume = "124",

pages = "139--40",

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T1 - A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters

AU - Caricasole, A A

AU - Ward, A

PY - 1993/2/14

Y1 - 1993/2/14

N2 - A luciferase-reporter plasmid vector has been constructed for the rapid subcloning and analysis of eukaryotic promoters. Identification of recombinants, when using this vector, is greatly facilitated by the retention of blue-white selection (alpha-complementation).

AB - A luciferase-reporter plasmid vector has been constructed for the rapid subcloning and analysis of eukaryotic promoters. Identification of recombinants, when using this vector, is greatly facilitated by the retention of blue-white selection (alpha-complementation).

KW - Animals

KW - Cloning, Molecular

KW - Coleoptera

KW - Genetic Vectors

KW - Introns

KW - Luciferases

KW - Plasmids

KW - Promoter Regions, Genetic

KW - Restriction Mapping

KW - Simian virus 40

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

UR - https://www.scopus.com/pages/publications/0027511394

U2 - 10.1016/0378-1119(93)90777-Z

DO - 10.1016/0378-1119(93)90777-Z

M3 - Article

C2 - 8382654

SN - 0378-1119

VL - 124

SP - 139

EP - 140

JO - Gene

JF - Gene

IS - 1

ER -

A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters

Abstract

Keywords

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