A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters

A A Caricasole, A Ward

Research output: Contribution to journalArticle

Abstract

A luciferase-reporter plasmid vector has been constructed for the rapid subcloning and analysis of eukaryotic promoters. Identification of recombinants, when using this vector, is greatly facilitated by the retention of blue-white selection (alpha-complementation).

Original languageEnglish
Pages (from-to)139-40
Number of pages2
JournalGene
Volume124
Issue number1
Publication statusPublished - 14 Feb 1993

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Luciferases
Plasmids

Keywords

  • Animals
  • Cloning, Molecular
  • Coleoptera
  • Genetic Vectors
  • Introns
  • Luciferases
  • Plasmids
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Simian virus 40
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters. / Caricasole, A A; Ward, A.

In: Gene, Vol. 124, No. 1, 14.02.1993, p. 139-40.

Research output: Contribution to journalArticle

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abstract = "A luciferase-reporter plasmid vector has been constructed for the rapid subcloning and analysis of eukaryotic promoters. Identification of recombinants, when using this vector, is greatly facilitated by the retention of blue-white selection (alpha-complementation).",
keywords = "Animals, Cloning, Molecular, Coleoptera, Genetic Vectors, Introns, Luciferases, Plasmids, Promoter Regions, Genetic, Restriction Mapping, Simian virus 40, Journal Article, Research Support, Non-U.S. Gov't",
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N2 - A luciferase-reporter plasmid vector has been constructed for the rapid subcloning and analysis of eukaryotic promoters. Identification of recombinants, when using this vector, is greatly facilitated by the retention of blue-white selection (alpha-complementation).

AB - A luciferase-reporter plasmid vector has been constructed for the rapid subcloning and analysis of eukaryotic promoters. Identification of recombinants, when using this vector, is greatly facilitated by the retention of blue-white selection (alpha-complementation).

KW - Animals

KW - Cloning, Molecular

KW - Coleoptera

KW - Genetic Vectors

KW - Introns

KW - Luciferases

KW - Plasmids

KW - Promoter Regions, Genetic

KW - Restriction Mapping

KW - Simian virus 40

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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JO - Gene

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