A hydrogel-based optical fibre fluorescent pH sensor for observing lung tumor tissue acidity

Jingjing Gong; Michael G. Tanner; Seshasailam Venkateswaran; James M. Stone; Yichuan Zhang; Mark Bradley

doi:10.1016/j.aca.2020.07.063

A hydrogel-based optical fibre fluorescent pH sensor for observing lung tumor tissue acidity

Jingjing Gong, Michael G. Tanner, Seshasailam Venkateswaran, James M. Stone, Yichuan Zhang, Mark Bradley

Department of Physics

Research output: Contribution to journal › Article › peer-review

54 Citations (SciVal)

232 Downloads (Pure)

Abstract

Technologies for measuring physiological parameters in vivo offer the possibility of the detection of disease and its progression due to the resulting changes in tissue pH, or temperature, etc. Here, a compact hydrogel-based optical fibre pH sensor was fabricated, in which polymer microarrays were utilized for the high-throughput discovery of an optimal matrix for pH indicator immobilization. The fabricated hydrogel-based probe responded rapidly to pH changes and demonstrated a good linear correlation within the physiological pH range (from 5.5 to 8.0) with a precision of 0.10 pH units. This miniature probe was validated by measuring pH across a whole ovine lung and allowed discrimination of tumorous and normal tissue, thus offering the potential for the rapid and accurate observation of tissue pH changes.

Original language	English
Pages (from-to)	136-143
Number of pages	8
Journal	Analytica Chimica Acta
Volume	1134
Early online date	7 Aug 2020
DOIs	https://doi.org/10.1016/j.aca.2020.07.063
Publication status	Published - 16 Oct 2020

Funding

Jingjing Gong acknowledges support from the Chinese Scholarship Council and The Henry Lester Trust. We thank the Engineering and Physical Sciences Research Council, UKRI , EPSRC , UK) Interdisciplinary Research Collaboration grants EP/K03197X/1 and EP/R005257/1 for funding. We acknowledge the use of the Cryo FIB/ SEM bought with the EPSRC grant EP/P030564/1 , Thomas Glen for help with image acquisition, Filip Angelov for tissue preparation, and Wikimedia Commons for the image of lung-simple diagram of lungs and trachea.

Keywords

Hydrogel-based pH sensor
In situ photo-polymerization
Lung tumor
Optical fibre
Polymer microarray

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

Access to Document

10.1016/j.aca.2020.07.063

Hydrogel final revised manuscriptAccepted author manuscript, 1.07 MBLicence: CC BY-NC-ND

Cite this

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title = "A hydrogel-based optical fibre fluorescent pH sensor for observing lung tumor tissue acidity",

abstract = "Technologies for measuring physiological parameters in vivo offer the possibility of the detection of disease and its progression due to the resulting changes in tissue pH, or temperature, etc. Here, a compact hydrogel-based optical fibre pH sensor was fabricated, in which polymer microarrays were utilized for the high-throughput discovery of an optimal matrix for pH indicator immobilization. The fabricated hydrogel-based probe responded rapidly to pH changes and demonstrated a good linear correlation within the physiological pH range (from 5.5 to 8.0) with a precision of 0.10 pH units. This miniature probe was validated by measuring pH across a whole ovine lung and allowed discrimination of tumorous and normal tissue, thus offering the potential for the rapid and accurate observation of tissue pH changes.",

keywords = "Hydrogel-based pH sensor, In situ photo-polymerization, Lung tumor, Optical fibre, Polymer microarray",

author = "Jingjing Gong and Tanner, \{Michael G.\} and Seshasailam Venkateswaran and Stone, \{James M.\} and Yichuan Zhang and Mark Bradley",

year = "2020",

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doi = "10.1016/j.aca.2020.07.063",

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AU - Gong, Jingjing

AU - Tanner, Michael G.

AU - Venkateswaran, Seshasailam

AU - Stone, James M.

AU - Zhang, Yichuan

AU - Bradley, Mark

PY - 2020/10/16

Y1 - 2020/10/16

N2 - Technologies for measuring physiological parameters in vivo offer the possibility of the detection of disease and its progression due to the resulting changes in tissue pH, or temperature, etc. Here, a compact hydrogel-based optical fibre pH sensor was fabricated, in which polymer microarrays were utilized for the high-throughput discovery of an optimal matrix for pH indicator immobilization. The fabricated hydrogel-based probe responded rapidly to pH changes and demonstrated a good linear correlation within the physiological pH range (from 5.5 to 8.0) with a precision of 0.10 pH units. This miniature probe was validated by measuring pH across a whole ovine lung and allowed discrimination of tumorous and normal tissue, thus offering the potential for the rapid and accurate observation of tissue pH changes.

AB - Technologies for measuring physiological parameters in vivo offer the possibility of the detection of disease and its progression due to the resulting changes in tissue pH, or temperature, etc. Here, a compact hydrogel-based optical fibre pH sensor was fabricated, in which polymer microarrays were utilized for the high-throughput discovery of an optimal matrix for pH indicator immobilization. The fabricated hydrogel-based probe responded rapidly to pH changes and demonstrated a good linear correlation within the physiological pH range (from 5.5 to 8.0) with a precision of 0.10 pH units. This miniature probe was validated by measuring pH across a whole ovine lung and allowed discrimination of tumorous and normal tissue, thus offering the potential for the rapid and accurate observation of tissue pH changes.

KW - Hydrogel-based pH sensor

KW - In situ photo-polymerization

KW - Lung tumor

KW - Optical fibre

KW - Polymer microarray

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A hydrogel-based optical fibre fluorescent pH sensor for observing lung tumor tissue acidity

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Fellowship - Next Generation Endoscopes

IRC Proteus II

Fellowship - Next Generation Endoscopes

Dynamic Mechanical Analyser (DMA)

Large chamber variable pressure scanning electron microscope (SEM)

Cite this

A hydrogel-based optical fibre fluorescent pH sensor for observing lung tumor tissue acidity

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Fellowship - Next Generation Endoscopes

IRC Proteus II

Fellowship - Next Generation Endoscopes

Equipment

Dynamic Mechanical Analyser (DMA)

Large chamber variable pressure scanning electron microscope (SEM)

Cite this