A Golgi and tonoplast localized S-acyl transferase is involved in cell expansion, cell division, vascular patterning and fertility in Arabidopsis

Baoxiu Qi, James Doughty, Richard Hooley

Research output: Contribution to journalArticle

29 Citations (Scopus)
148 Downloads (Pure)

Abstract

Summary: S-acylation of eukaryotic proteins is the reversible attachment of palmitic or stearic acid to cysteine residues, catalysed by protein S-acyl transferases that share an Asp-His-His-Cys (DHHC) motif. Previous evidence suggests that in Arabidopsis S-acylation is involved in the control of cell size, polarity and the growth of pollen tubes and root hairs. Using a combination of yeast genetics, biochemistry, cell biology and loss of function genetics the roles of a member of the protein S-acyl transferase PAT family, AtPAT10 (At3g51390), have been explored. In keeping with its role as a PAT, AtPAT10 auto-S-acylates, and partially complements the yeast akr1 PAT mutant, and this requires Cys192 of the DHHC motif. In Arabidopsis AtPAT10 is localized in the Golgi stack, trans-Golgi network/early endosome and tonoplast. Loss-of-function mutants have a pleiotropic phenotype involving cell expansion and division, vascular patterning, and fertility that is rescued by wild-type AtPAT10 but not by catalytically inactive AtPAT10C192A. This supports the hypothesis that AtPAT10 is functionally independent of the other Arabidopsis PATs. Our findings demonstrate a growing importance of protein S-acylation in plants, and reveal a Golgi and tonoplast located S-acylation mechanism that affects a range of events during growth and development in Arabidopsis.
Original languageEnglish
Pages (from-to)444-456
Number of pages13
JournalNew Phytologist
Volume200
Issue number2
Early online date25 Jun 2013
DOIs
Publication statusPublished - Oct 2013

Keywords

  • Arabidopsis
  • DHHC-PAT
  • vascular patterning
  • trans-Golgi network
  • tonoplast
  • protein S-acylation
  • dwarfism

Fingerprint Dive into the research topics of 'A Golgi and tonoplast localized S-acyl transferase is involved in cell expansion, cell division, vascular patterning and fertility in Arabidopsis'. Together they form a unique fingerprint.

  • Projects

    Cite this