A gene-by-gene approach to bacterial population genomics: Whole genome MLST of Campylobacter

Samuel K. Sheppard, Keith A. Jolley, Martin C J Maiden

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Campylobacteriosis remains a major human public health problem world-wide. Genetic analyses of Campylobacter isolates, and particularly molecular epidemiology, have been central to the study of this disease, particularly the characterization of Campylobacter genotypes isolated from human infection, farm animals, and retail food. These studies have demonstrated that Campylobacter populations are highly structured, with distinct genotypes associated with particular wild or domestic animal sources, and that chicken meat is the most likely source of most human infection in countries such as the UK. The availability of multiple whole genome sequences from Campylobacter isolates presents the prospect of identifying those genes or allelic variants responsible for host-association and increased human disease risk, but the diversity of Campylobacter genomes present challenges for such analyses. We present a gene-by-gene approach for investigating the genetic basis of phenotypes in diverse bacteria such as Campylobacter, implemented with the BIGSDB software on the pubMLST.org/campylobacter website.

Original languageEnglish
Pages (from-to)261-277
Number of pages17
JournalGenes
Volume3
Issue number2
DOIs
Publication statusPublished - 12 Apr 2012

Keywords

  • Campylobacter coli
  • Campylobacter jejuni
  • Campylobacteriosis
  • Genome analysis
  • Next generation sequencing
  • Whole genome sequencing

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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