Abstract
Recently, we reported the effect of mild alkaline treatment on the structure and enzymatic hydrolysis of waxy maize starch. Here, we have used extended-q small-angle neutron scattering (SANS) to examine the extent to which this treatment causes structural changes on even greater length scales — to yield a complete picture from the nanometre to micron — including on the lamellar distribution and larger-scale structural features of the same starch. For this A-type polymorph starch containing mostly amylopectin, we found the changes caused by NaOH solution treatment (0.1% or 0.5% (w/v)) were confined mainly to double helices and crystallites, whereas the lamellar and supramolecular structures were conserved. Although the overall lamellar ordering was reduced in the 0.5% (w/v) alkaline treatment for 6 days, further treatment until 12 days could restore the overall lamellar ordering. Despite changes in starch double helical order and crystallites, there were minimal changes at larger length scales (e.g., blocklets and growth rings). This combination of methods reveals the extent of structural changes occurring as a result of mild alkaline treatment of waxy maize starch; it also provides insight into the rational design of granular starch products with varied functionality via hydrolytic control.
| Original language | English |
|---|---|
| Pages (from-to) | 133-142 |
| Number of pages | 10 |
| Journal | Food Hydrocolloids |
| Volume | 95 |
| DOIs | |
| Publication status | Published - 1 Oct 2019 |
Funding
The authors would like to acknowledge the National Natural Science Foundation of China (NSFC) (Project Nos. 31701637 and 31801582), the Fundamental Research Funds for the Central Universities (Project No. 2662016QD008), the China Postdoctoral Science Foundation (Project No. 2018M642865), the Department of Education, Hubei Province (Q20181407), and the Doctoral Startup Foundation, Hubei University of Technology (BSQD2016024). B. Zhang also would like to thank for the supports from the Young Elite Scientists Sponsorship Program of the China Association for Science and Technology, the Chutian Scholars Program of Hubei Province, and the Shishan Scholars Program of Huazhong Agricultural University. F. Xie acknowledges the support from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 798225. D. Wang thanks for the support from the Australian Research Council under a Discovery Project (Project No. DP190101734). This research was undertaken on the QUOKKA instrument at the OPAL reactor at the Australian Nuclear Science and Technology Organisation (ANSTO), New South Wales, Australia. The authors would like to acknowledge the National Natural Science Foundation of China (NSFC) (Project Nos. 31701637 and 31801582 ), the Fundamental Research Funds for the Central Universities (Project No. 2662016QD008 ), the China Postdoctoral Science Foundation (Project No. 2018M642865 ), the Department of Education, Hubei Province ( Q20181407 ), and the Doctoral Startup Foundation, Hubei University of Technology ( BSQD2016024 ). B. Zhang also would like to thank for the supports from the Young Elite Scientists Sponsorship Program of the China Association for Science and Technology , the Chutian Scholars Program of Hubei Province , and the Shishan Scholars Program of Huazhong Agricultural University . F. Xie acknowledges the support from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 798225 . D. Wang thanks for the support from the Australian Research Council under a Discovery Project (Project No. DP190101734 ). This research was undertaken on the QUOKKA instrument at the OPAL reactor at the Australian Nuclear Science and Technology Organisation (ANSTO), New South Wales, Australia.
| Funders | Funder number |
|---|---|
| Chutian Scholars Program of Hubei Province | |
| Doctoral Startup Foundation | |
| H2020 Marie Skłodowska-Curie Actions | |
| China Association for Science and Technology | |
| Horizon 2020 Framework Programme | |
| H2020 Marie Skłodowska-Curie Actions | 798225 |
| European Commission | |
| Hubei Provincial Department of Education | Q20181407 |
| Australian Research Council | DP190101734 |
| National Natural Science Foundation of China | 31701637, 31801582 |
| China Postdoctoral Science Foundation | 2018M642865 |
| Hubei University of Technology | BSQD2016024 |
| Huazhong Agricultural University | |
| Fundamental Research Funds for the Central Universities | 2662016QD008 |
Keywords
- Double helices
- Extended-q small-angle neutron scattering
- Lamellae
- Large-scale structure
- SANS
- Starch
ASJC Scopus subject areas
- Food Science
- General Chemistry
- General Chemical Engineering