A fluorescent chemodosimeter for live-cell monitoring of aqueous sulfides

Shiguo Wang, Suying Xu, Gaofei Hu, Xilin Bai, Tony D. James, Leyu Wang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Aqueous sulfides are emerging signaling agents implicated in various pathological and physiological processes. The development of sensitive and selective methods for the sensing of these sulfides is therefore very important. Herein, we report that the as-synthesized 1-oxo-1H-phenalene-2,3-dicarbonitrile (OPD) compound provides promising fluorescent properties and unique reactive properties toward aqueous sulfides. It was found that OPD showed high selectivity and sensitivity toward Na2S over thiols and other inorganic sulfur compounds through a sulfide involved reaction which was confirmed by high-resolution mass spectroscopy (HRMS) and nuclear magnetic resonance (NMR) results. The fluorescence intensity increases linearly with sulfide concentration in the range of 1.0-30 μM with a limit of detection of 52 nM. This novel fluorescent probe was further exploited for the fluorescence imaging sensing of aqueous sulfide in HeLa cells.

Original languageEnglish
Pages (from-to)1434-1439
Number of pages6
JournalAnalytical Chemistry
Volume88
Issue number2
DOIs
Publication statusPublished - 19 Jan 2016

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