A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro

Zhiyuan Hu, Rory L Arrowsmith, James A Tyson, Vincenzo Mirabello, Haobo Ge, Ian M Eggleston, Stanley W Botchway, Dan Pantos, Sofia I Pascu

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Abstract

We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

Original languageEnglish
Pages (from-to)6901-6904
Number of pages4
JournalChemical Communications
Volume51
Issue number32
Early online date16 Jan 2015
DOIs
Publication statusPublished - 25 Apr 2015

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Integrins
Tryptophan
Peptides
Amino acids
Imaging techniques
Amino Acids
Fluorescence
Confocal microscopy
Fluorescence microscopy
Photons
Cells
arginyl-glycyl-aspartic acid
naphthalenediimide

Cite this

A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro. / Hu, Zhiyuan; Arrowsmith, Rory L; Tyson, James A; Mirabello, Vincenzo; Ge, Haobo; Eggleston, Ian M; Botchway, Stanley W; Pantos, Dan; Pascu, Sofia I.

In: Chemical Communications, Vol. 51, No. 32, 25.04.2015, p. 6901-6904.

Research output: Contribution to journalArticle

Hu, Zhiyuan ; Arrowsmith, Rory L ; Tyson, James A ; Mirabello, Vincenzo ; Ge, Haobo ; Eggleston, Ian M ; Botchway, Stanley W ; Pantos, Dan ; Pascu, Sofia I. / A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro. In: Chemical Communications. 2015 ; Vol. 51, No. 32. pp. 6901-6904.
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AU - Hu, Zhiyuan

AU - Arrowsmith, Rory L

AU - Tyson, James A

AU - Mirabello, Vincenzo

AU - Ge, Haobo

AU - Eggleston, Ian M

AU - Botchway, Stanley W

AU - Pantos, Dan

AU - Pascu, Sofia I

PY - 2015/4/25

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AB - We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

UR - http://dx.doi.org/10.1039/c4cc08265f

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DO - 10.1039/c4cc08265f

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JF - Chemical communications (Cambridge, England)

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