A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro

Zhiyuan Hu; Rory L Arrowsmith; James A Tyson; Vincenzo Mirabello; Haobo Ge; Ian M Eggleston; Stanley W Botchway; Dan Pantos; Sofia I Pascu

doi:10.1039/c4cc08265f

A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin α_vβ₃in vitro

Zhiyuan Hu, Rory L Arrowsmith, James A Tyson, Vincenzo Mirabello, Haobo Ge, Ian M Eggleston, Stanley W Botchway, Dan Pantos, Sofia I Pascu

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Abstract

We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

Original language	English
Pages (from-to)	6901-6904
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	32
Early online date	16 Jan 2015
DOIs	https://doi.org/10.1039/c4cc08265f
Publication status	Published - 25 Apr 2015

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10.1039/c4cc08265f

12. A fluorescent RGD ChemCommunAccepted author manuscript, 930 KB

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title = "A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro",

abstract = "We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.",

author = "Zhiyuan Hu and Arrowsmith, {Rory L} and Tyson, {James A} and Vincenzo Mirabello and Haobo Ge and Eggleston, {Ian M} and Botchway, {Stanley W} and Dan Pantos and Pascu, {Sofia I}",

year = "2015",

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doi = "10.1039/c4cc08265f",

language = "English",

volume = "51",

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journal = "Chemical Communications",

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AU - Hu, Zhiyuan

AU - Arrowsmith, Rory L

AU - Tyson, James A

AU - Mirabello, Vincenzo

AU - Ge, Haobo

AU - Eggleston, Ian M

AU - Botchway, Stanley W

AU - Pantos, Dan

AU - Pascu, Sofia I

PY - 2015/4/25

Y1 - 2015/4/25

N2 - We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

AB - We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

UR - http://dx.doi.org/10.1039/c4cc08265f

U2 - 10.1039/c4cc08265f

DO - 10.1039/c4cc08265f

M3 - Article

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SN - 1359-7345

VL - 51

SP - 6901

EP - 6904

JO - Chemical Communications

JF - Chemical Communications

IS - 32

ER -

A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin α_vβ₃in vitro

Abstract

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Dan Pantos

Sofia Pascu, FRSC

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A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro

Abstract

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Dan Pantos

Sofia Pascu, FRSC

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A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin α_vβ₃in vitro