A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro

Zhiyuan Hu, Rory L Arrowsmith, James A Tyson, Vincenzo Mirabello, Haobo Ge, Ian M Eggleston, Stanley W Botchway, Dan Pantos, Sofia I Pascu

Research output: Contribution to journal › Article

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Abstract

We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

Original languageEnglish
Pages (from-to)6901-6904
Number of pages4
JournalChemical Communications
Volume51
Issue number32
Early online date16 Jan 2015
DOIs
Publication statusPublished - 25 Apr 2015

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Integrins
Tryptophan
Peptides
Amino acids
Imaging techniques
Amino Acids
Fluorescence
Confocal microscopy
Fluorescence microscopy
Photons
Cells
arginyl-glycyl-aspartic acid
naphthalenediimide

Cite this

A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro. / Hu, Zhiyuan; Arrowsmith, Rory L; Tyson, James A; Mirabello, Vincenzo; Ge, Haobo; Eggleston, Ian M; Botchway, Stanley W; Pantos, Dan; Pascu, Sofia I.

In: Chemical Communications, Vol. 51, No. 32, 25.04.2015, p. 6901-6904.

Research output: Contribution to journal › Article

Hu, Z, Arrowsmith, RL, Tyson, JA, Mirabello, V, Ge, H, Eggleston, IM, Botchway, SW, Pantos, D & Pascu, SI 2015, 'A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro', Chemical Communications, vol. 51, no. 32, pp. 6901-6904. https://doi.org/10.1039/c4cc08265f
Hu Z, Arrowsmith RL, Tyson JA, Mirabello V, Ge H, Eggleston IM et al. A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro. Chemical Communications. 2015 Apr 25;51(32):6901-6904. https://doi.org/10.1039/c4cc08265f
Hu, Zhiyuan ; Arrowsmith, Rory L ; Tyson, James A ; Mirabello, Vincenzo ; Ge, Haobo ; Eggleston, Ian M ; Botchway, Stanley W ; Pantos, Dan ; Pascu, Sofia I. / A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro. In: Chemical Communications. 2015 ; Vol. 51, No. 32. pp. 6901-6904.
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abstract = "We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.",
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AU - Hu, Zhiyuan

AU - Arrowsmith, Rory L

AU - Tyson, James A

AU - Mirabello, Vincenzo

AU - Ge, Haobo

AU - Eggleston, Ian M

AU - Botchway, Stanley W

AU - Pantos, Dan

AU - Pascu, Sofia I

PY - 2015/4/25

Y1 - 2015/4/25

N2 - We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

AB - We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

UR - http://dx.doi.org/10.1039/c4cc08265f

U2 - 10.1039/c4cc08265f

DO - 10.1039/c4cc08265f

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EP - 6904

JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

SN - 1359-7345

IS - 32

ER -

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