Abstract
Throughout embryonic development, macrophages not only constitute the first line of defense against infection but also help to sculpt organs and tissues of the embryo by removing dead cells and secreting extracellular matrix components. Key to their function is the ability of embryonic macrophages to migrate and disperse throughout the embryo. Yet despite these important developmental functions, little is known about the molecular mechanisms underlying embryonic macrophage migration in vivo. Integrins are key regulators of many of the adult macrophage responses, but their role in embryonic macrophages remains poorly characterized. Here, we have used Drosophila macrophages (haemocytes) as a model system to address the role of integrins during embryonic macrophage dispersal in vivo. We show that the main βPS integrin, myospheroid, affects haemocyte migration in two ways; by shaping the 3-dimensional environment in which haemocytes migrate and by regulating the migration of haemocytes themselves. Live imaging reveals a requirement for myospheroid within haemocytes for coordinating the microtubule and actin dynamics, and in enabling haemocyte developmental dispersal, contact repulsion and inflammatory migration towards wounds.
| Original language | English |
|---|---|
| Pages (from-to) | 3475-3484 |
| Number of pages | 10 |
| Journal | Journal of Cell Science |
| Volume | 126 |
| Issue number | 15 |
| Early online date | 23 May 2013 |
| DOIs | |
| Publication status | Published - 1 Aug 2013 |
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Keywords
- integrins
- macrophage
- migration
Cite this
A dual role for the βPS integrin myospheroid in mediating Drosophila embryonic macrophage migration. / Comber, Kate ; Huelsmann, Sven; Evans, Iwan; Sánchez-Sánchez, Besaid J.; Chalmers, Andrew; Reuter, Rolf; Wood, Will; Martín-Bermudo, Maria D.
In: Journal of Cell Science, Vol. 126, No. 15, 01.08.2013, p. 3475-3484.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A dual role for the βPS integrin myospheroid in mediating Drosophila embryonic macrophage migration
AU - Comber, Kate
AU - Huelsmann, Sven
AU - Evans, Iwan
AU - Sánchez-Sánchez, Besaid J.
AU - Chalmers, Andrew
AU - Reuter, Rolf
AU - Wood, Will
AU - Martín-Bermudo, Maria D.
PY - 2013/8/1
Y1 - 2013/8/1
N2 - Throughout embryonic development, macrophages not only constitute the first line of defense against infection but also help to sculpt organs and tissues of the embryo by removing dead cells and secreting extracellular matrix components. Key to their function is the ability of embryonic macrophages to migrate and disperse throughout the embryo. Yet despite these important developmental functions, little is known about the molecular mechanisms underlying embryonic macrophage migration in vivo. Integrins are key regulators of many of the adult macrophage responses, but their role in embryonic macrophages remains poorly characterized. Here, we have used Drosophila macrophages (haemocytes) as a model system to address the role of integrins during embryonic macrophage dispersal in vivo. We show that the main βPS integrin, myospheroid, affects haemocyte migration in two ways; by shaping the 3-dimensional environment in which haemocytes migrate and by regulating the migration of haemocytes themselves. Live imaging reveals a requirement for myospheroid within haemocytes for coordinating the microtubule and actin dynamics, and in enabling haemocyte developmental dispersal, contact repulsion and inflammatory migration towards wounds.
AB - Throughout embryonic development, macrophages not only constitute the first line of defense against infection but also help to sculpt organs and tissues of the embryo by removing dead cells and secreting extracellular matrix components. Key to their function is the ability of embryonic macrophages to migrate and disperse throughout the embryo. Yet despite these important developmental functions, little is known about the molecular mechanisms underlying embryonic macrophage migration in vivo. Integrins are key regulators of many of the adult macrophage responses, but their role in embryonic macrophages remains poorly characterized. Here, we have used Drosophila macrophages (haemocytes) as a model system to address the role of integrins during embryonic macrophage dispersal in vivo. We show that the main βPS integrin, myospheroid, affects haemocyte migration in two ways; by shaping the 3-dimensional environment in which haemocytes migrate and by regulating the migration of haemocytes themselves. Live imaging reveals a requirement for myospheroid within haemocytes for coordinating the microtubule and actin dynamics, and in enabling haemocyte developmental dispersal, contact repulsion and inflammatory migration towards wounds.
KW - integrins
KW - macrophage
KW - migration
UR - http://www.scopus.com/inward/record.url?scp=84883387982&partnerID=8YFLogxK
U2 - 10.1242/jcs.129700
DO - 10.1242/jcs.129700
M3 - Article
VL - 126
SP - 3475
EP - 3484
JO - Journal of Cell Science
JF - Journal of Cell Science
SN - 0021-9533
IS - 15
ER -