A dual-responsive Golgi-targeting probe for the simultaneous fluorescence detection of SO2 and viscosity in food specimens and live cells

Ze-Yi Lian; Pan-Dong Mao; Kun-Sheng Jin; Wei Na Wu; Lin-Yan Bian; Yuan Wang; Yun Chang Fan; Zhi Hong Xu; Tony James

doi:10.1016/j.snb.2025.137933

A dual-responsive Golgi-targeting probe for the simultaneous fluorescence detection of SO2 and viscosity in food specimens and live cells

Ze-Yi Lian, Pan-Dong Mao, Kun-Sheng Jin, Wei Na Wu, Lin-Yan Bian, Yuan Wang, Yun Chang Fan, Zhi Hong Xu, Tony James

Research output: Contribution to journal › Article › peer-review

Abstract

The Golgi apparatus is responsible for regulating the transport of proteins. Research has revealed that an increase in viscosity and sulfur dioxide (SO ₂) levels in the Golgi are associated with many diseases. Unfortunately, no probes currently exist to simultaneously measure variations in viscosity and SO ₂ in the Golgi apparatus. With this research, we modified a coumarin-cyanoacetamide probe (1) with a sulfanilamide moiety for targeting the Golgi apparatus. Probe 1 could respond to viscosity increases by exhibiting fluorescence enhancements at 590 nm due to the inhibition of intramolecular rotation. Moreover, the nucleophilic attack of the C[dbnd]C bond by HSO ₃ ⁻ resulted in creating a conjugate addition product that generated a blue emission band at 480 nm. Significantly, this probe can identify HSO ₃ ⁻ in real water samples and food specimens, as well as monitor HSO ₃ ⁻ levels and changes of viscosity in the Golgi apparatus of plants, live cells, and zebrafish.

Original language	English
Article number	137933
Journal	Sensors and Actuators B: Chemical
Volume	441
Early online date	17 May 2025
DOIs	https://doi.org/10.1016/j.snb.2025.137933
Publication status	E-pub ahead of print - 17 May 2025

Data Availability Statement

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.

Acknowledgements

We would also like to acknowledge the support of (www.home-for-researchers.com) who helped reduce textual overlap with previously published research. After using this service, the authors have reviewed and edited the content as needed and take full responsibility for the content of the publication.

Funding

This work was supported by the National Natural Science Foundation of China (No. 21907023 ), the Education Department of Henan Province (No. 24A150010 ), the Natural Science Foundation of Henan Province (No. 242300420190 , 252300420243 , and 252300420793 ), the Fundamental Research Funds for the Universities of Henan Province (No. NSFRF240809 and NSFRF230402 ), the Double World-Class Project of Henan Polytechnic University (No. AQ20230745 and AQ20230754 ), the Innovative Research Team of Henan Polytechnic University ( T2023\u20136 ), the Key Program of Xuchang University ( 2022CXTD001 ), the University of Bath, and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University ( 2020ZD01 ). We would also like to acknowledge the support of ( www.home-for-researchers.com ) who helped reduce textual overlap with previously published research. After using this service, the authors have reviewed and edited the content as needed and take full responsibility for the content of the publication.

Funders	Funder number
University of Bath
Education Department of Henan Province	24A150010
Natural Science Foundation of Henan Province	242300420190, 252300420793, 252300420243
Key Programme	2022CXTD001
National Natural Science Foundation of China	21907023
Fundamental Research Funds for the Universities of Henan Province	NSFRF240809, NSFRF230402
Henan Polytechnic University	T2023–6, AQ20230745, AQ20230754
Henan Normal University	2020ZD01

Keywords

Bioimaging
Fluorescent probe
Food specimens
SO
Viscosity

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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Cite this

Lian, Z.-Y., Mao, P.-D., Jin, K.-S., Wu, W. N., Bian, L.-Y., Wang, Y., Fan, Y. C., Xu, Z. H., & James, T. (2025). A dual-responsive Golgi-targeting probe for the simultaneous fluorescence detection of SO2 and viscosity in food specimens and live cells. Sensors and Actuators B: Chemical, 441, Article 137933. Advance online publication. https://doi.org/10.1016/j.snb.2025.137933

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abstract = "The Golgi apparatus is responsible for regulating the transport of proteins. Research has revealed that an increase in viscosity and sulfur dioxide (SO 2) levels in the Golgi are associated with many diseases. Unfortunately, no probes currently exist to simultaneously measure variations in viscosity and SO 2 in the Golgi apparatus. With this research, we modified a coumarin-cyanoacetamide probe (1) with a sulfanilamide moiety for targeting the Golgi apparatus. Probe 1 could respond to viscosity increases by exhibiting fluorescence enhancements at 590 nm due to the inhibition of intramolecular rotation. Moreover, the nucleophilic attack of the C[dbnd]C bond by HSO 3 − resulted in creating a conjugate addition product that generated a blue emission band at 480 nm. Significantly, this probe can identify HSO 3 − in real water samples and food specimens, as well as monitor HSO 3 − levels and changes of viscosity in the Golgi apparatus of plants, live cells, and zebrafish.",

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AU - Wu, Wei Na

AU - Bian, Lin-Yan

AU - Wang, Yuan

AU - Fan, Yun Chang

AU - Xu, Zhi Hong

AU - James, Tony

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A dual-responsive Golgi-targeting probe for the simultaneous fluorescence detection of SO2 and viscosity in food specimens and live cells

Abstract

Data Availability Statement

Acknowledgements

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this