A dual functional peptide carrying in vitro selected catalytic and binding activities

Liping Zhu, Wei Wang, Haixu Zhao, Muye Xu, Seiichi Tada, Takanori Uzawa, Mingzhe Liu, Yoshihiro Ito

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
156 Downloads (Pure)

Abstract

When minimal functional sequences are used, it is possible to integrate multiple functions on a single peptide chain, like a "single stroke drawing". Here a dual functional peptide was designed by combining in vitro selected catalytic and binding activities. For catalytic activity, we performed in vitro selection for a peptide aptamer binding to hemin by using ribosome display and isolated a peptide that had peroxidase activity in the presence of hemin. By combining the selected catalytic peptide with a peptide antigen, which can be recognized by an antibody, an enzyme-antibody conjugate-like peptide was obtained. This study demonstrates a successful strategy to create dual functionalized peptide chains for use in immunoassays.

Original languageEnglish
Pages (from-to)9808-9812
Number of pages5
JournalOrganic and Biomolecular Chemistry
Volume13
Issue number38
Early online date4 Aug 2015
DOIs
Publication statusPublished - Oct 2015

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Biochemistry
  • Medicine(all)

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