A coumarin-based probe with far-red emission for the ratiometric detection of peroxynitrite in the mitochondria of living cells and mice

Yu Man Tian; Hui Juan Lai; Wei Na Wu; Xiao Lei Zhao; Yuan Wang; Yun Chang Fan; Zhi Hong Xu; Tony D. James

doi:10.1016/j.talanta.2024.127272

A coumarin-based probe with far-red emission for the ratiometric detection of peroxynitrite in the mitochondria of living cells and mice

Yu Man Tian, Hui Juan Lai, Wei Na Wu, Xiao Lei Zhao, Yuan Wang, Yun Chang Fan, Zhi Hong Xu, Tony D. James

Research output: Contribution to journal › Article › peer-review

Abstract

Peroxynitrite (ONOO⁻) is a transient and reactive oxidant with significant roles in numerous biological processes. Research has established a correlation between excessive mitochondrial ONOO⁻ production and various diseases. As such we developed a mitochondria-targeting, fluorescence-based ratiometric probe using a boronate group for ONOO⁻ recognition and coumarin as the fluorophore. The probe exhibited a 615 nm far-red emission, and a new fluorescence emission at 475 nm developed upon adding ONOO⁻.The probe possessed high sensitivity and selectivity for ONOO⁻ detection with distinct ratiometric fluorescent output and a low detection limit of 11 nM. Significantly, Probe 1 could monitor ONOO⁻ level fluctuations in biological systems due to its superior spectral properties and low toxicity. Notably, probe 1 has been effectively used for imaging in live HepG2 cells, zebrafish, Arabidopsis thaliana, and liver injury mouse tissues.

Original language	English
Article number	127272
Journal	Talanta
Volume	284
Early online date	22 Nov 2024
DOIs	https://doi.org/10.1016/j.talanta.2024.127272
Publication status	E-pub ahead of print - 22 Nov 2024

Data Availability Statement

All data supporting this research is available in the manuscript or supporting information

Funding

This research was supported in part by the National Natural Science Foundation of China (No. 21907023), the Natural Science Foundation of Henan Province (No. 242300420190), the Education Department of Henan Province (No. 24A150010), the Double World-Class Project of Henan Polytechnic University (No. AQ20230745 and AQ20230754), the Fundamental Research Funds for the Universities of Henan Province (No. NSFRF240809 and NSFRF230402), Innovative Research Team of Henan Polytechnic University (T2023-6), and Key Program of Xuchang University (2022CXTD001). T.D.J. thanks the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support.

Keywords

Biological imaging
Coumarin
Mitochondria
Peroxynitrite
Ratiometric fluorescent probe

ASJC Scopus subject areas

Analytical Chemistry

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title = "A coumarin-based probe with far-red emission for the ratiometric detection of peroxynitrite in the mitochondria of living cells and mice",

abstract = "Peroxynitrite (ONOO−) is a transient and reactive oxidant with significant roles in numerous biological processes. Research has established a correlation between excessive mitochondrial ONOO− production and various diseases. As such we developed a mitochondria-targeting, fluorescence-based ratiometric probe using a boronate group for ONOO− recognition and coumarin as the fluorophore. The probe exhibited a 615 nm far-red emission, and a new fluorescence emission at 475 nm developed upon adding ONOO−.The probe possessed high sensitivity and selectivity for ONOO− detection with distinct ratiometric fluorescent output and a low detection limit of 11 nM. Significantly, Probe 1 could monitor ONOO− level fluctuations in biological systems due to its superior spectral properties and low toxicity. Notably, probe 1 has been effectively used for imaging in live HepG2 cells, zebrafish, Arabidopsis thaliana, and liver injury mouse tissues.",

keywords = "Biological imaging, Coumarin, Mitochondria, Peroxynitrite, Ratiometric fluorescent probe",

author = "Tian, {Yu Man} and Lai, {Hui Juan} and Wu, {Wei Na} and Zhao, {Xiao Lei} and Yuan Wang and Fan, {Yun Chang} and Xu, {Zhi Hong} and James, {Tony D.}",

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doi = "10.1016/j.talanta.2024.127272",

language = "English",

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T1 - A coumarin-based probe with far-red emission for the ratiometric detection of peroxynitrite in the mitochondria of living cells and mice

AU - Tian, Yu Man

AU - Lai, Hui Juan

AU - Wu, Wei Na

AU - Zhao, Xiao Lei

AU - Wang, Yuan

AU - Fan, Yun Chang

AU - Xu, Zhi Hong

AU - James, Tony D.

PY - 2024/11/22

Y1 - 2024/11/22

N2 - Peroxynitrite (ONOO−) is a transient and reactive oxidant with significant roles in numerous biological processes. Research has established a correlation between excessive mitochondrial ONOO− production and various diseases. As such we developed a mitochondria-targeting, fluorescence-based ratiometric probe using a boronate group for ONOO− recognition and coumarin as the fluorophore. The probe exhibited a 615 nm far-red emission, and a new fluorescence emission at 475 nm developed upon adding ONOO−.The probe possessed high sensitivity and selectivity for ONOO− detection with distinct ratiometric fluorescent output and a low detection limit of 11 nM. Significantly, Probe 1 could monitor ONOO− level fluctuations in biological systems due to its superior spectral properties and low toxicity. Notably, probe 1 has been effectively used for imaging in live HepG2 cells, zebrafish, Arabidopsis thaliana, and liver injury mouse tissues.

AB - Peroxynitrite (ONOO−) is a transient and reactive oxidant with significant roles in numerous biological processes. Research has established a correlation between excessive mitochondrial ONOO− production and various diseases. As such we developed a mitochondria-targeting, fluorescence-based ratiometric probe using a boronate group for ONOO− recognition and coumarin as the fluorophore. The probe exhibited a 615 nm far-red emission, and a new fluorescence emission at 475 nm developed upon adding ONOO−.The probe possessed high sensitivity and selectivity for ONOO− detection with distinct ratiometric fluorescent output and a low detection limit of 11 nM. Significantly, Probe 1 could monitor ONOO− level fluctuations in biological systems due to its superior spectral properties and low toxicity. Notably, probe 1 has been effectively used for imaging in live HepG2 cells, zebrafish, Arabidopsis thaliana, and liver injury mouse tissues.

KW - Biological imaging

KW - Coumarin

KW - Mitochondria

KW - Peroxynitrite

KW - Ratiometric fluorescent probe

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DO - 10.1016/j.talanta.2024.127272

M3 - Article

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SN - 0039-9140

VL - 284

JO - Talanta

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ER -

A coumarin-based probe with far-red emission for the ratiometric detection of peroxynitrite in the mitochondria of living cells and mice

Abstract

Data Availability Statement

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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