Abstract
We have developed and refined a rapid, reliable method for the evaluation of attachment and proliferation of ovine meniscal chondrocytes in microcarrier culture. Assays measuring both mitochondrial activity, using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and MTS [3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium], and DNA synthesis with a PicoGreen assay were compared. The MTT assay was the most sensitive at lower cell concns. and enabled accurate assessment of cell proliferation over 14 day culture. [on SciFinder (R)]
| Original language | English |
|---|---|
| Pages (from-to) | 1451-1455 |
| Number of pages | 5 |
| Journal | Biotechnology Letters |
| Volume | 27 |
| Issue number | 19 |
| Publication status | Published - 2005 |
Keywords
- Carriers (microcarriers
- Animal tissue culture
- ANST (Analytical study) (comparison of colorimetric and DNA quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture)
- colorimetry mitochondria DNA chondrocyte culture microcarrier MTT MTS
- comparison of colorimetric and DNA quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture)
- Chondrocyte (meniscal
- Cytodex 1
- DNA formation
- Colorimetry
- Mitochondria
- Ovis aries (comparison of colorimetric and DNA quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture)
- 3
- 2
- DNA Role
- ANT (Analyte)
- Cell proliferation