A Comparison of Colorimetric and DNA Quantification Assays for the Assessment of Meniscal Fibrochondrocyte Proliferation in Microcarrier Culture

Moreica B Pabbruwe, Karina Stewart, Julian B Chaudhuri

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

We have developed and refined a rapid, reliable method for the evaluation of attachment and proliferation of ovine meniscal chondrocytes in microcarrier culture. Assays measuring both mitochondrial activity, using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and MTS [3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium], and DNA synthesis with a PicoGreen assay were compared. The MTT assay was the most sensitive at lower cell concns. and enabled accurate assessment of cell proliferation over 14 day culture. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)1451-1455
Number of pages5
JournalBiotechnology Letters
Volume27
Issue number19
Publication statusPublished - 2005

Keywords

  • Carriers (microcarriers
  • Animal tissue culture
  • ANST (Analytical study) (comparison of colorimetric and DNA quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture)
  • colorimetry mitochondria DNA chondrocyte culture microcarrier MTT MTS
  • comparison of colorimetric and DNA quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture)
  • Chondrocyte (meniscal
  • Cytodex 1
  • DNA formation
  • Colorimetry
  • Mitochondria
  • Ovis aries (comparison of colorimetric and DNA quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture)
  • 3
  • 2
  • DNA Role
  • ANT (Analyte)
  • Cell proliferation

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