A comparative genomics study of Staphylococcus epidermidis from orthopedic device-related infections correlated with patient outcome

Virginia Post, Llinos G Harris, Mario Morgenstern, Leonardos Mageiros, Matthew D. Hitchings, Guillaume Méric, Ben Pascoe, Samuel K Sheppard, R Geoff Richards, T Fintan Moriarty

Research output: Contribution to journalArticle

21 Citations (Scopus)
91 Downloads (Pure)

Abstract

Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic device-related infections (ODRIs). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome of the infected patient. S. epidermidis isolates were collected from 104 patients with ODRI. Their clinical outcome was evaluated, after an average of 26 months, as either "cured" or "not cured". The isolates were tested for antibiotic susceptibility and biofilm formation. Whole genome sequencing was performed on all isolates and genomic variation was related to features associated with "cured" and "not cured". Strong biofilm formation and aminoglycoside resistance were associated with a "not cured" outcome (p = 0.031 and p < 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the "not cured" group. These included: the biofilm-associated bhp gene; the antiseptic resistance qacA gene, the cassette chromosome recombinase encoding genes ccrA and ccrB and IS256-like transposase. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in S. epidermidis ODRI. Whole genome sequencing identified specific genes associated with a "not cured" outcome that should be validated in future studies.

Original languageEnglish
Article number00881-17
Pages (from-to)3089-3103
JournalJournal of Clinical Microbiology
Volume55
Issue number10
Early online date9 Aug 2017
DOIs
Publication statusPublished - 1 Oct 2017

Fingerprint

Staphylococcus epidermidis
Genomics
Orthopedics
Equipment and Supplies
Biofilms
Infection
Genes
Genome
Transposases
Recombinases
Local Anti-Infective Agents
Aminoglycosides
Microbial Drug Resistance
Chromosomes
Anti-Bacterial Agents

Keywords

  • Journal Article

Cite this

Post, V., Harris, L. G., Morgenstern, M., Mageiros, L., Hitchings, M. D., Méric, G., ... Moriarty, T. F. (2017). A comparative genomics study of Staphylococcus epidermidis from orthopedic device-related infections correlated with patient outcome. Journal of Clinical Microbiology, 55(10), 3089-3103. [00881-17]. https://doi.org/10.1128/JCM.00881-17

A comparative genomics study of Staphylococcus epidermidis from orthopedic device-related infections correlated with patient outcome. / Post, Virginia; Harris, Llinos G; Morgenstern, Mario; Mageiros, Leonardos; Hitchings, Matthew D.; Méric, Guillaume; Pascoe, Ben; Sheppard, Samuel K; Richards, R Geoff; Moriarty, T Fintan.

In: Journal of Clinical Microbiology, Vol. 55, No. 10, 00881-17, 01.10.2017, p. 3089-3103.

Research output: Contribution to journalArticle

Post, V, Harris, LG, Morgenstern, M, Mageiros, L, Hitchings, MD, Méric, G, Pascoe, B, Sheppard, SK, Richards, RG & Moriarty, TF 2017, 'A comparative genomics study of Staphylococcus epidermidis from orthopedic device-related infections correlated with patient outcome', Journal of Clinical Microbiology, vol. 55, no. 10, 00881-17, pp. 3089-3103. https://doi.org/10.1128/JCM.00881-17
Post, Virginia ; Harris, Llinos G ; Morgenstern, Mario ; Mageiros, Leonardos ; Hitchings, Matthew D. ; Méric, Guillaume ; Pascoe, Ben ; Sheppard, Samuel K ; Richards, R Geoff ; Moriarty, T Fintan. / A comparative genomics study of Staphylococcus epidermidis from orthopedic device-related infections correlated with patient outcome. In: Journal of Clinical Microbiology. 2017 ; Vol. 55, No. 10. pp. 3089-3103.
@article{22e54ea9f3534335a1029b859b8527d9,
title = "A comparative genomics study of Staphylococcus epidermidis from orthopedic device-related infections correlated with patient outcome",
abstract = "Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic device-related infections (ODRIs). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome of the infected patient. S. epidermidis isolates were collected from 104 patients with ODRI. Their clinical outcome was evaluated, after an average of 26 months, as either {"}cured{"} or {"}not cured{"}. The isolates were tested for antibiotic susceptibility and biofilm formation. Whole genome sequencing was performed on all isolates and genomic variation was related to features associated with {"}cured{"} and {"}not cured{"}. Strong biofilm formation and aminoglycoside resistance were associated with a {"}not cured{"} outcome (p = 0.031 and p < 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the {"}not cured{"} group. These included: the biofilm-associated bhp gene; the antiseptic resistance qacA gene, the cassette chromosome recombinase encoding genes ccrA and ccrB and IS256-like transposase. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in S. epidermidis ODRI. Whole genome sequencing identified specific genes associated with a {"}not cured{"} outcome that should be validated in future studies.",
keywords = "Journal Article",
author = "Virginia Post and Harris, {Llinos G} and Mario Morgenstern and Leonardos Mageiros and Hitchings, {Matthew D.} and Guillaume M{\'e}ric and Ben Pascoe and Sheppard, {Samuel K} and Richards, {R Geoff} and Moriarty, {T Fintan}",
note = "Copyright {\circledC} 2017 American Society for Microbiology.",
year = "2017",
month = "10",
day = "1",
doi = "10.1128/JCM.00881-17",
language = "English",
volume = "55",
pages = "3089--3103",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - A comparative genomics study of Staphylococcus epidermidis from orthopedic device-related infections correlated with patient outcome

AU - Post, Virginia

AU - Harris, Llinos G

AU - Morgenstern, Mario

AU - Mageiros, Leonardos

AU - Hitchings, Matthew D.

AU - Méric, Guillaume

AU - Pascoe, Ben

AU - Sheppard, Samuel K

AU - Richards, R Geoff

AU - Moriarty, T Fintan

N1 - Copyright © 2017 American Society for Microbiology.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic device-related infections (ODRIs). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome of the infected patient. S. epidermidis isolates were collected from 104 patients with ODRI. Their clinical outcome was evaluated, after an average of 26 months, as either "cured" or "not cured". The isolates were tested for antibiotic susceptibility and biofilm formation. Whole genome sequencing was performed on all isolates and genomic variation was related to features associated with "cured" and "not cured". Strong biofilm formation and aminoglycoside resistance were associated with a "not cured" outcome (p = 0.031 and p < 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the "not cured" group. These included: the biofilm-associated bhp gene; the antiseptic resistance qacA gene, the cassette chromosome recombinase encoding genes ccrA and ccrB and IS256-like transposase. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in S. epidermidis ODRI. Whole genome sequencing identified specific genes associated with a "not cured" outcome that should be validated in future studies.

AB - Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic device-related infections (ODRIs). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome of the infected patient. S. epidermidis isolates were collected from 104 patients with ODRI. Their clinical outcome was evaluated, after an average of 26 months, as either "cured" or "not cured". The isolates were tested for antibiotic susceptibility and biofilm formation. Whole genome sequencing was performed on all isolates and genomic variation was related to features associated with "cured" and "not cured". Strong biofilm formation and aminoglycoside resistance were associated with a "not cured" outcome (p = 0.031 and p < 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the "not cured" group. These included: the biofilm-associated bhp gene; the antiseptic resistance qacA gene, the cassette chromosome recombinase encoding genes ccrA and ccrB and IS256-like transposase. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in S. epidermidis ODRI. Whole genome sequencing identified specific genes associated with a "not cured" outcome that should be validated in future studies.

KW - Journal Article

UR - https://doi.org/10.1128/JCM.00881-17

U2 - 10.1128/JCM.00881-17

DO - 10.1128/JCM.00881-17

M3 - Article

C2 - 28794175

VL - 55

SP - 3089

EP - 3103

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 10

M1 - 00881-17

ER -