A compact holographic optical tweezers instrument

G.M. Gibson, R.W. Bowman, A. Linnenberger, M. Dienerowitz, D.B. Phillips, D.M. Carberry, M.J. Miles, M.J. Padgett

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Abstract

Holographic optical tweezers have found many applications including the construction of complex micron-scale 3D structures and the control of tools and probes for position, force, and viscosity measurement. We have developed a compact, stable, holographic optical tweezers instrument which can be easily transported and is compatible with a wide range of microscopy techniques, making it a valuable tool for collaborative research. The instrument measures approximately 30×30×35 cm and is designed around a custom inverted microscope, incorporating a fibre laser operating at 1070 nm. We designed the control software to be easily accessible for the non-specialist, and have further improved its ease of use with a multi-touch iPad interface. A high-speed camera allows multiple trapped objects to be tracked simultaneously. We demonstrate that the compact instrument is stable to 0.5 nm for a 10 s measurement time by plotting the Allan variance of the measured position of a trapped 2 μm silica bead. We also present a range of objects that have been successfully manipulated.
Original languageEnglish
Article number113107
JournalReview of Scientific Instruments
Volume83
Issue number11
DOIs
Publication statusPublished - Nov 2012

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Gibson, G. M., Bowman, R. W., Linnenberger, A., Dienerowitz, M., Phillips, D. B., Carberry, D. M., ... Padgett, M. J. (2012). A compact holographic optical tweezers instrument. Review of Scientific Instruments, 83(11), [113107]. https://doi.org/10.1063/1.4768303