Abstract
The use of biodiesel-derived glycerol as a carbon source for microbial oil production is a biorefinery engineering strategy that aims to reduce the glycerol surplus and make the microbial oil process more cost-effective. In this work, glycerol was used as the sole carbon source for the cultivation of the oleaginous yeast Rhodotorula glutinis along with only yeast extract as a nutrient supply and without pH control. Shake-flask cultivations showed that the specific growth rate and glycerol consumption of Rhodotorula glutinis were higher at lower glycerol concentrations (≤40 g L−1), while higher C/N atom ratios enhanced oil content. The present study extends the knowledge on the influence of the aeration rate and oxygen supply in cellular growth rate and microbial oil production, providing a wiser use of glycerol as an attempt to further reduce process costs. Cultivations at different air flow rates were performed in a 2 L bioreactor and showed that a low aeration rate of 0.5 L min−1 gave the best glycerol and nitrogen uptake rates, resulting in the highest growth (5.3 g L−1) and oil mass fraction (33% of the dry cell weight). A further 68% increase in cellular growth (16.8 g L−1) and a 34% oil mass fraction of the dry cell weight was achieved after applying a feeding strategy targeting combined growth and oil production.
Original language | English |
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Pages (from-to) | 113-122 |
Number of pages | 10 |
Journal | Biomass and Bioenergy |
Volume | 89 |
Early online date | 30 Jan 2016 |
DOIs | |
Publication status | Published - 30 Jun 2016 |
Keywords
- Aeration rate
- C/N ratio
- Fed-batch
- Oleaginous yeast
- Oxygen uptake rate
- Single cell oil
ASJC Scopus subject areas
- Forestry
- Renewable Energy, Sustainability and the Environment
- Agronomy and Crop Science
- Waste Management and Disposal