A bifunctional coumarin/phenanthridine-fused probe for the detection of mitochondrial peroxynitrite in live cells, Arabidopsis thaliana, zebrafish, and mice

Xi Chen; Hui Juan Lai; Wei Na Wu; Xiao Lei Zhao; Yun Chang Fan; Lin-Yan Bian; Yuan Wang; Zhi Hong Xu; Tony James

doi:10.1016/j.snb.2025.138066

A bifunctional coumarin/phenanthridine-fused probe for the detection of mitochondrial peroxynitrite in live cells, Arabidopsis thaliana, zebrafish, and mice

Xi Chen, Hui Juan Lai, Wei Na Wu, Xiao Lei Zhao, Yun Chang Fan, Lin-Yan Bian, Yuan Wang, Zhi Hong Xu, Tony James

Research output: Contribution to journal › Article › peer-review

Abstract

Aberrant peroxynitrite (ONOO−) may damage a wide array of biomolecules, causing cellular dysfunctions that are closely associated with multiple oxidative stress-related diseases. Therefore, detecting ONOO− levels in physiological and pathological processes is of great significance. With this research, a coumarin/phenanthridine-fused probe 1 was synthesized in high yield, and exhibited high selectivity toward ONOO− for both fluorescence and UV–vis channels in DMSO/HEPES (v/v, 4/6, pH=7.4) solution. The probe exhibited a near-infrared (NIR) emission at 710 nm with a large Stokes shift of 200 nm, which was selectively quenched by adding ONOO−. The probe was used to track ONOO− in live cells, Arabidopsis thaliana, zebrafish, and mice.

Original language	English
Article number	138066
Journal	Sensors and Actuators B: Chemical
Volume	442
Early online date	28 May 2025
DOIs	https://doi.org/10.1016/j.snb.2025.138066
Publication status	E-pub ahead of print - 28 May 2025

Data Availability Statement

All data supporting this research is available from the manuscript and supporting information.

Funding

This work was supported in part by the National Natural Science Foundation of China (No. 21907023 ), the Natural Science Foundation of Henan Province (No. 252300420243 ), the Education Department of Henan Province (No. 24A150010 ), the Double World-Class Project of Henan Polytechnic University (No. AQ20230745 and AQ20230754 ), the Fundamental Research Funds for the Universities of Henan Province (No. NSFRF240809 and NSFRF230402 ), Innovative Research Team of Henan Polytechnic University ( T2023\u20136 ), and Key Program of Xuchang University ( 2022CXTD001 ). T.D.J. wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University ( 2020ZD01 ) for support.

Funders	Funder number
University of Bath
National Natural Science Foundation of China	21907023
Natural Science Foundation of Henan Province	252300420243
Fundamental Research Funds for the Universities of Henan Province	NSFRF240809, T2023–6, NSFRF230402
Xuchang University	2022CXTD001
Henan Polytechnic University	AQ20230745, AQ20230754
Education Department of Henan Province	24A150010
Henan Normal University	2020ZD01

Keywords

Bio-imaging
Coumarin/phenanthridine
Fluorescent probe
Mitochondria-targeted
Peroxynitrite

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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Cite this

Chen, X., Lai, H. J., Wu, W. N., Zhao, X. L., Fan, Y. C., Bian, L.-Y., Wang, Y., Xu, Z. H., & James, T. (2025). A bifunctional coumarin/phenanthridine-fused probe for the detection of mitochondrial peroxynitrite in live cells, Arabidopsis thaliana, zebrafish, and mice. Sensors and Actuators B: Chemical, 442, Article 138066. Advance online publication. https://doi.org/10.1016/j.snb.2025.138066

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A bifunctional coumarin/phenanthridine-fused probe for the detection of mitochondrial peroxynitrite in live cells, Arabidopsis thaliana, zebrafish, and mice

Abstract

Data Availability Statement

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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