5-Formylcytosine can be a stable DNA modification in mammals

Martin Bachman; Santiago Uribe-Lewis; Xiaoping Yang; Heather E. Burgess; Mario Iurlaro; Wolf Reik; Adele Murrell; Shankar Balasubramanian

doi:10.1038/nchembio.1848

5-Formylcytosine can be a stable DNA modification in mammals

Martin Bachman, Santiago Uribe-Lewis, Xiaoping Yang, Heather E. Burgess, Mario Iurlaro, Wolf Reik, Adele Murrell, Shankar Balasubramanian

Department of Life Sciences

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Abstract

5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.

Original language	English
Pages (from-to)	555-557
Number of pages	3
Journal	Nature Chemical Biology
Volume	11
Issue number	8
Early online date	22 Jun 2015
DOIs	https://doi.org/10.1038/nchembio.1848
Publication status	Published - 13 Aug 2015

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Adele Murrell
- A.M.Murrellbath.acuk
- Centre for Therapeutic Innovation
- Centre for Mathematical Biology - Co-Director
- Department of Life Sciences - Head of Department
Person: Research & Teaching

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title = "5-Formylcytosine can be a stable DNA modification in mammals",

abstract = "5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.",

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AU - Bachman, Martin

AU - Uribe-Lewis, Santiago

AU - Yang, Xiaoping

AU - Burgess, Heather E.

AU - Iurlaro, Mario

AU - Reik, Wolf

AU - Murrell, Adele

AU - Balasubramanian, Shankar

PY - 2015/8/13

Y1 - 2015/8/13

N2 - 5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.

AB - 5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.

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DO - 10.1038/nchembio.1848

M3 - Article

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JF - Nature Chemical Biology

SN - 1552-4450

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5-Formylcytosine can be a stable DNA modification in mammals

Abstract

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Adele Murrell

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