5-Formylcytosine can be a stable DNA modification in mammals

Martin Bachman, Santiago Uribe-Lewis, Xiaoping Yang, Heather E. Burgess, Mario Iurlaro, Wolf Reik, Adele Murrell, Shankar Balasubramanian

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Abstract

5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.
Original languageEnglish
Pages (from-to)555-557
Number of pages3
JournalNature Chemical Biology
Volume11
Issue number8
Early online date22 Jun 2015
DOIs
Publication statusPublished - 1 Aug 2015

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Mammals
DNA
Isotope Labeling
5-formylcytosine

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Bachman, M., Uribe-Lewis, S., Yang, X., Burgess, H. E., Iurlaro, M., Reik, W., ... Balasubramanian, S. (2015). 5-Formylcytosine can be a stable DNA modification in mammals. Nature Chemical Biology, 11(8), 555-557. https://doi.org/10.1038/nchembio.1848

5-Formylcytosine can be a stable DNA modification in mammals. / Bachman, Martin; Uribe-Lewis, Santiago; Yang, Xiaoping; Burgess, Heather E.; Iurlaro, Mario; Reik, Wolf; Murrell, Adele; Balasubramanian, Shankar.

In: Nature Chemical Biology, Vol. 11, No. 8, 01.08.2015, p. 555-557.

Research output: Contribution to journal › Article

Bachman, M, Uribe-Lewis, S, Yang, X, Burgess, HE, Iurlaro, M, Reik, W, Murrell, A & Balasubramanian, S 2015, '5-Formylcytosine can be a stable DNA modification in mammals', Nature Chemical Biology, vol. 11, no. 8, pp. 555-557. https://doi.org/10.1038/nchembio.1848
Bachman M, Uribe-Lewis S, Yang X, Burgess HE, Iurlaro M, Reik W et al. 5-Formylcytosine can be a stable DNA modification in mammals. Nature Chemical Biology. 2015 Aug 1;11(8):555-557. https://doi.org/10.1038/nchembio.1848
Bachman, Martin ; Uribe-Lewis, Santiago ; Yang, Xiaoping ; Burgess, Heather E. ; Iurlaro, Mario ; Reik, Wolf ; Murrell, Adele ; Balasubramanian, Shankar. / 5-Formylcytosine can be a stable DNA modification in mammals. In: Nature Chemical Biology. 2015 ; Vol. 11, No. 8. pp. 555-557.
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