5-Formylcytosine can be a stable DNA modification in mammals

Martin Bachman; Santiago Uribe-Lewis; Xiaoping Yang; Heather E. Burgess; Mario Iurlaro; Wolf Reik; Adele Murrell; Shankar Balasubramanian

doi:10.1038/nchembio.1848

5-Formylcytosine can be a stable DNA modification in mammals

Martin Bachman, Santiago Uribe-Lewis, Xiaoping Yang, Heather E. Burgess, Mario Iurlaro, Wolf Reik, Adele Murrell, Shankar Balasubramanian

Department of Biology & Biochemistry

Research output: Contribution to journal › Article › peer-review

145 Citations (Scopus)

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Abstract

5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.

Original language	English
Pages (from-to)	555-557
Number of pages	3
Journal	Nature Chemical Biology
Volume	11
Issue number	8
Early online date	22 Jun 2015
DOIs	https://doi.org/10.1038/nchembio.1848
Publication status	Published - 13 Aug 2015

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Adele Murrell
- A.M.Murrell@bath.ac.uk
- Department of Biology & Biochemistry - Professor
- Centre for Therapeutic Innovation
Person: Research & Teaching

Cite this

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abstract = "5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.",

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AU - Reik, Wolf

AU - Murrell, Adele

AU - Balasubramanian, Shankar

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AB - 5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.

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