Projects per year
Abstract
Sonic hedgehog (Shh) signalling controls integrated specification of digit pattern and growth in the chick wing but downstream gene networks remain to be unravelled. We analysed 3D expression patterns of genes encoding cell cycle regulators using Optical Projection Tomography. Hierarchical clustering of spatial matrices of gene expression revealed a dorsal layer of the wing bud, in which almost all genes were expressed, and that genes encoding positive cell cycle regulators had similar expression patterns while those of N-myc and CyclinD2 were distinct but closely related. We compared these patterns computationally with those of genes implicated in digit specification and Ptch1, 50 genes in total. Nineteen genes have similar posterior expression to Ptch1, including Hoxd13, Sall1, Hoxd11, and Bmp2, all likely Gli targets in mouse limb, and cell cycle genes, N-myc, CyclinD2. We suggest that these genes contribute to a network integrating digit specification and growth in response to Shh.
Original language | English |
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Pages (from-to) | 1278-1288 |
Number of pages | 11 |
Journal | Developmental Dynamics |
Volume | 240 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2011 |
Keywords
- cell cycle genes
- chick
- Hoxd13
- Ptch1
- OPT
- limb
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Dive into the research topics of '3D expression patterns of cell cycle genes in the developing chick wing and comparison with expression patterns of genes implicated in digit specification'. Together they form a unique fingerprint.Projects
- 3 Finished
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Patterning and Growth in Embryonic Limb Development
Tickle, C. (PI)
1/01/09 → 31/12/10
Project: Research council
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A Three-Dimensional Atlas of Gene Expression during chick Development with Cross Comparisons to the Mouse
Tickle, C. (PI)
Biotechnology and Biological Sciences Research Council
1/12/08 → 31/12/10
Project: Research council
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Signalling in Vertebrate Limb Development
Tickle, C. (PI) & Downie, H. (CoI)
1/09/07 → 31/12/08
Project: Research council