3D expression patterns of cell cycle genes in the developing chick wing and comparison with expression patterns of genes implicated in digit specification

Monique Welten, Gordana Pavlovska, Yu Chen, Y Teruoka, M Fisher, Fiona Bangs, Matthew Towers, Cheryll Tickle

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Sonic hedgehog (Shh) signalling controls integrated specification of digit pattern and growth in the chick wing but downstream gene networks remain to be unravelled. We analysed 3D expression patterns of genes encoding cell cycle regulators using Optical Projection Tomography. Hierarchical clustering of spatial matrices of gene expression revealed a dorsal layer of the wing bud, in which almost all genes were expressed, and that genes encoding positive cell cycle regulators had similar expression patterns while those of N-myc and CyclinD2 were distinct but closely related. We compared these patterns computationally with those of genes implicated in digit specification and Ptch1, 50 genes in total. Nineteen genes have similar posterior expression to Ptch1, including Hoxd13, Sall1, Hoxd11, and Bmp2, all likely Gli targets in mouse limb, and cell cycle genes, N-myc, CyclinD2. We suggest that these genes contribute to a network integrating digit specification and growth in response to Shh.
Original languageEnglish
Pages (from-to)1278-1288
Number of pages11
JournalDevelopmental Dynamics
Volume240
Issue number5
DOIs
Publication statusPublished - May 2011

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cdc Genes
Gene Expression
Genes
Hedgehogs
Optical Tomography
Gene Regulatory Networks
Growth
Cluster Analysis
Cell Cycle
Extremities

Keywords

  • cell cycle genes
  • chick
  • Hoxd13
  • Ptch1
  • OPT
  • limb

Cite this

3D expression patterns of cell cycle genes in the developing chick wing and comparison with expression patterns of genes implicated in digit specification. / Welten, Monique; Pavlovska, Gordana; Chen, Yu; Teruoka, Y; Fisher, M; Bangs, Fiona; Towers, Matthew; Tickle, Cheryll.

In: Developmental Dynamics, Vol. 240, No. 5, 05.2011, p. 1278-1288.

Research output: Contribution to journalArticle

Welten, Monique ; Pavlovska, Gordana ; Chen, Yu ; Teruoka, Y ; Fisher, M ; Bangs, Fiona ; Towers, Matthew ; Tickle, Cheryll. / 3D expression patterns of cell cycle genes in the developing chick wing and comparison with expression patterns of genes implicated in digit specification. In: Developmental Dynamics. 2011 ; Vol. 240, No. 5. pp. 1278-1288.
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