2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2

Carmen Domene, Martine I. Abboud, Tom E McAllister, Ivanhoe K. H. Leung, Rasheduzzaman Chowdhury, Christian Jorgensen, Jasmin Mecinović, Kerstin Lippl, Rebecca L Hancock, Richard J Hopkinson, Akane Kawamura, Timothy D.W. Claridge, Christopher J. Schofield

Research output: Contribution to journalArticle

  • 2 Citations

Abstract

Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(II)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ~50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding
LanguageEnglish
Pages3130-3133
JournalChemical Communications
Volume54
Issue number25
Early online date9 Mar 2018
DOIs
StatusPublished - 28 Mar 2018

Fingerprint

Prolyl Hydroxylases
Hydroxylation
Animals
Enzymes
Degradation
alpha-ketoglutaric acid

Cite this

Domene, C., Abboud, M. I., McAllister, T. E., Leung, I. K. H., Chowdhury, R., Jorgensen, C., ... Schofield, C. J. (2018). 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2. DOI: 10.1039/C8CC00387D

2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2. / Domene, Carmen; Abboud, Martine I.; McAllister, Tom E; Leung, Ivanhoe K. H.; Chowdhury, Rasheduzzaman; Jorgensen, Christian; Mecinović, Jasmin; Lippl, Kerstin; Hancock, Rebecca L; Hopkinson, Richard J; Kawamura, Akane; Claridge, Timothy D.W.; Schofield, Christopher J.

In: Chemical Communications, Vol. 54, No. 25, 28.03.2018, p. 3130-3133.

Research output: Contribution to journalArticle

Domene, C, Abboud, MI, McAllister, TE, Leung, IKH, Chowdhury, R, Jorgensen, C, Mecinović, J, Lippl, K, Hancock, RL, Hopkinson, RJ, Kawamura, A, Claridge, TDW & Schofield, CJ 2018, '2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2' Chemical Communications, vol. 54, no. 25, pp. 3130-3133. DOI: 10.1039/C8CC00387D
Domene C, Abboud MI, McAllister TE, Leung IKH, Chowdhury R, Jorgensen C et al. 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2. Chemical Communications. 2018 Mar 28;54(25):3130-3133. Available from, DOI: 10.1039/C8CC00387D
Domene, Carmen ; Abboud, Martine I. ; McAllister, Tom E ; Leung, Ivanhoe K. H. ; Chowdhury, Rasheduzzaman ; Jorgensen, Christian ; Mecinović, Jasmin ; Lippl, Kerstin ; Hancock, Rebecca L ; Hopkinson, Richard J ; Kawamura, Akane ; Claridge, Timothy D.W. ; Schofield, Christopher J./ 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2. In: Chemical Communications. 2018 ; Vol. 54, No. 25. pp. 3130-3133
@article{68a0af2ff00346b485a9bebfd92c9c65,
title = "2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2",
abstract = "Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(II)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ~50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding",
author = "Carmen Domene and Abboud, {Martine I.} and McAllister, {Tom E} and Leung, {Ivanhoe K. H.} and Rasheduzzaman Chowdhury and Christian Jorgensen and Jasmin Mecinović and Kerstin Lippl and Hancock, {Rebecca L} and Hopkinson, {Richard J} and Akane Kawamura and Claridge, {Timothy D.W.} and Schofield, {Christopher J.}",
year = "2018",
month = "3",
day = "28",
doi = "10.1039/C8CC00387D",
language = "English",
volume = "54",
pages = "3130--3133",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "25",

}

TY - JOUR

T1 - 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2

AU - Domene,Carmen

AU - Abboud,Martine I.

AU - McAllister,Tom E

AU - Leung,Ivanhoe K. H.

AU - Chowdhury,Rasheduzzaman

AU - Jorgensen,Christian

AU - Mecinović,Jasmin

AU - Lippl,Kerstin

AU - Hancock,Rebecca L

AU - Hopkinson,Richard J

AU - Kawamura,Akane

AU - Claridge,Timothy D.W.

AU - Schofield,Christopher J.

PY - 2018/3/28

Y1 - 2018/3/28

N2 - Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(II)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ~50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding

AB - Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(II)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ~50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding

U2 - 10.1039/C8CC00387D

DO - 10.1039/C8CC00387D

M3 - Article

VL - 54

SP - 3130

EP - 3133

JO - Chemical Communications

T2 - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 25

ER -