Structural Studies on Human Angiotensin-1 Converting Enzyme (ACE) and the Design of Novel Domain-Specific Inhibitors

Project: Research council

Project Details

Description

Angiotensin-I converting enzyme [ACE, which contains two domains (N and C)] inhibitors are widely used to treat cardiovascular diseases, including high blood pressure, heart failure, coronary artery disease, fibrosis and kidney failure. However, current-generation ACE inhibitors, which were developed in the 1970?s and 1980?s, are hampered by common side effects. While there are many ACE inhibitors on the market that block both domains, there are no drugs that selectively inhibit the N domain and thereby accrue the advantages of reducing fibrosis and inflammation in the heart, kidney and lung, without the concomitant side effects induced by blockade of the C domain.. This underscores the importance of the determination of the 3D structure of ACE and the design of 2nd generation ACE-inhibitor complex/s that are safer and more effective. Our success in the determination of the crystal structure of human testis ACE (equivalent to the C domain of somatic ACE) and the N-domain of somatic ACE using X-ray crystallography have provided the platform for true structure-based design of ACE inhibitors. This is a significant breakthrough in terms of the structural biology of the protease and, more importantly, the mechanism of ACE inhibition. This paves the way for a more rigorous approach exploiting the differences between the domains through a structure based drug design approach of novel domain-selective inhibitors. Our proposed experiments are directed at structural study of the full-length somatic ACE and crystal structures of complexes of ACE with domain selective inhibitors combining basic and translational research on a important medical problem.
StatusFinished
Effective start/end date1/10/1130/09/14

Funding

  • Medical Research Council

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  • Research Output

    A new high-resolution crystal structure of the Drosophila melanogaster angiotensin converting enzyme homologue, AnCE

    Harrison, C. & Acharya, K. R., Jan 2015, In : FEBS Open Bio. 5, p. 661-667

    Research output: Contribution to journalArticle

  • 1 Citation (Scopus)

    Structural basis of Ac-SDKP hydrolysis by Angiotensin-I converting enzyme

    Masuyer, G., Douglas, R. G., Sturrock, E. D. & Acharya, K. R., 25 Sep 2015, In : Scientific Reports. 5, 13742.

    Research output: Contribution to journalArticle

    Open Access
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  • 7 Citations (Scopus)
    104 Downloads (Pure)

    Absence of cell surface expression of human ACE leads to perinatal death

    Michaud, A., Acharya, K. R., Masuyer, G., Quenech'du, N., Gribouval, O., Morinière, V., Gubler, M-C. & Corvol, P., Mar 2014, In : Human Molecular Genetics. 23, 6, p. 1479-1491 13 p.

    Research output: Contribution to journalArticle

  • 5 Citations (Scopus)