APPLICATION OF MESOSCALE TECHNOLOGY FOR THE MEASUREMENT OF M ULTIPLE BIOMARKERS TO EXPLORE MOLECULAR AND CELLULAR PROCESS

Project: Research council

Project Details

Description

Analysis of molecular and cellular events is crucial in modern life sciences research, for a better understanding of (i) fundamental biological processes, (ii) the changes that characterise associated disease states, and (iii) the development of therapeutics. This research proposal brings together seven research areas, spanning the regulation of the immune and nervous systems, haemopoiesis, tissue development and regeneration and cell metabolism that share a common theme of wanting to utilise sensitive, quantitative and high throughput state-of-the-art instrumentation to analyse a range of cellular mechanisms. This instrument is the Mesoscale Discovery (MSD) SECTOR Imager (SI) 6000 which offers a sensitive, adaptable platform with which to measure several analytes in cell or cell-derived samples. The technology relies on the use of chemically tagged antibodies that recognize the analytes of choice. When bound to their target, the Abs emit light upon electrochemical stimulation which is measured and quantified. The main features provided by the instrument are: - the ability to accurately quantify activation of specific signals; - it facilitates detection of multiple signals simultaneously. In situations where cell samples may be limiting, information on several different signalling molecules can be generated simultaneously. - the system allows for more rapid analysis, - provides platform for developing our own custom made assays for future applications.
StatusFinished
Effective start/end date17/04/0816/04/09

Funding

  • BBSRC

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  • Research Output

    Genetic ablation of PI3Kγ results in defective IL-17RA signalling in T lymphocytes and increased IL-17 levels

    Harris, S. J., Ciuclan, L., Finan, P. M., Wymann, M. P., Walker, C., Westwick, J., Ward, S. G. & Thomas, M. J., Dec 2012, In : European Journal of Immunology. 42, 12, p. 3394-3404 11 p.

    Research output: Contribution to journalArticle

  • 11 Citations (Scopus)

    Evidence that the lipid phosphatase SHIP-1 regulates T lymphocyte morphology and motility

    Harris, S. J., Parry, R. V., Foster, J. G., Blunt, M. D., Wang, A., Marelli-Berg, F., Westwick, J. & Ward, S. G., 15 Apr 2011, In : The Journal of Immunology. 186, 8, p. 4936-4945 10 p.

    Research output: Contribution to journalArticle

  • 17 Citations (Scopus)