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Research interests

Research over-view

The question at the centre of our research is how two gametes - sperm and egg - transform into an embryo when they combine in mammalian fertilisation: the gamete-to-embryo transition.  This is a fundamental process with major implications for the regulation of cellular potency and broad translational potential in the areas of infertility and stem cell biology.  We integrate embryological and molecular approaches developed by us in a mouse model.  Using piezo-actuated micromanipulation, we can achieve high rates of transgenesis via a method we developed (published in Science), generate offspring from 'dead' sperm and perform nuclear transfer cloning; at Bath, we produced England's first cloned mouse; indeed, this was the first adult to be cloned from an adult cell in any species (<>).  Recently, we demonstrated the birth of offspring via a distinctive epigenetic pathway when sperm are injected into parthenogenetic embryos.  This finding overturns a centuries-old dogma established by Karl Ernst von Baer that animals develop from eggs and challenges the model of lineage specification proposed by twentieth century biologist, Conrad Waddington ('Waddington's Canal').  Our analytical methods include fluorescence imaging and sub-cellular transcriptomics.  We are developing next-generation genome editing, nanobiology and stem cell approaches and hold that regenerative medicine will remain impracticable without a thorough mechanistic understanding of the establishment of totipotency during the gamete-to-embryo transition.



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Research Output 1998 2018

Intracytoplasmic sperm injection (ICSI): applications and insights

Suzuki, T. & Perry, A. C. F. 4 Jan 2018 Intracytoplasmic Sperm Injection. Palermo, G. D. & Sills, E. S. (eds.). Cham, Switzerland: Springer International Publishing AG, p. 169-181

Research output: Chapter in Book/Report/Conference proceedingChapter

intracytoplasmic sperm injection
fertilization (reproduction)
4 Citations

Assisted reproductive technologies to prevent human mitochondrial disease transmission

Greenfield, A., Braude, P., Flinter, F., Lovell-Badge, R., Ogilvie, C. & Perry, A. 14 Dec 2017 In : Nature Biotechnology. 35, p. 1059-1068 10 p.

Research output: Contribution to journalArticle

Mitochondrial Diseases
Assisted Reproductive Techniques
Mitochondrial DNA
14 Citations

DNA methylation dynamics in mouse preimplantation embryos revealed by mass spectrometry

Okamoto, Y., Yoshida, N., Suzuki, T., Shimozawa, N., Asami, M., Matsuda, T., Kojima, N., Perry, A. C. F. & Takada, T. 11 Jan 2016 In : Scientific Reports. 6, 19134

Research output: Contribution to journalArticle

Open Access
DNA Methylation
Mass Spectrometry
5 Citations

Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes

Suzuki, T., Asami, M., Hoffmann, M., Lu, X., Gužvić, M., Klein, C. A. & Perry, A. C. F. 13 Sep 2016 In : Nature Communications. 7, 12676

Research output: Contribution to journalArticle

Open Access
53 Citations

CRISPR germline engineering-the community speaks

Bosley, K. , Botchan, M. , Bredenoord, A. , Carroll, D. , Charo, R. A. , Charpentier, E. , Cohen, R. , Corn, J. , Doudna, J. , Feng, G. , Greely, H. , Isasi, R. , Ji, W. , Kim, J-S. , Knoppers, B. , Lanphier, E. , Li, J. , Lovell-Badge, R. , Martin, G. S. , Moreno, J. & 6 others Naldini, L., Pera, M., Perry, A., Venter, J. C., Zhang, F. & Zhou, Q. 12 May 2015 In : Nature Biotechnology. 33, 5, p. 478-486

Research output: Contribution to journalComment/debate