Photo of Susan Crennell

Susan Crennell

Dr

  • 4 SOUTH 0.26

20022021

Research output per year

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Personal profile

Research interests

Current Research

Glycoside hydrolases, enzymes which cleave glycoside bonds to produce two smaller sugars, are widespread in nature and have correspondingly broad use in biotechnology. Cellulases, glycoside hydrolases that break down cellulose, are used in a broad range of industrial processes, for example clarifying fruit juices, textile processing and a rapidly expanding interest in the possibility of extracting ethanol from waste products of the food industry (stalks, husks etc.) to use as a renewable biofuel.

Thermostability: As part of the Centre for Extremophile Research, I am interested in the structural basis for protein thermostability, comparing proteins from hyperthermophilic organisms to their homologues from organisms growing at room temperature or below.

A recent glycoside hydrolase example is the biotechnologically-important cellulase from Rhodothermus marinus which has a half life of more than 2.5 hours at 90°C, (research in collaboration with Eva Nordberg Karlsson, Lund University). In complex with inhibitors or flash-frozen with substrate, X-ray crystal structures of this cellulase have revealed details of substrate binding mechanisms as well as thermophilic adaptation.  

This enzyme shows a number of thermophilic adaptations, the most obvious being a large increase in the number of surface electrostatic interactions (ion pairs) over mesophilic counterparts.

Complementary techniques used to investigate structure and function include X-ray scattering (e.g. for the structure of Rhodothermus marinus xylanase, see the CER pages) and computational analysis. We have used the University of Houston Brownian Dynamics program (UHBD) to quantify the contribution of ion pairs to thermostability of citrate synthase and are testing these predictions in the laboratory by altering the number of ion pairs using site directed mutagenesis.

Pathogenesis: Studies of other glycoside hydrolases such as cell-wall-degrading enzymes secreted by Stagonospora nodorum, a wheat pathogen, understanding of which will aid disease control (in collaboration with Prof Richard Cooper) and enzymes with potential for intervention in human disease (in collaboration with Dr Andrew Watts, Pharmacy & Pharmacology) are at a less advanced stage.

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Projects

  • Research Output

    Crystal structure of an inferred ancestral bacterial pyruvate decarboxylase

    Buddrus, L., Andrews, E. S. V., Leak, D., Danson, M., Arcus, V. L. & Crennell, S., 1 Mar 2018, In : Acta Crystallographica Section F:Structural Biology Communications. 74, 3, p. 179-186 8 p.

    Research output: Contribution to journalArticle

    Open Access
  • 2 Citations (Scopus)

    Distinctive phosphoinositide- A nd Ca 2+-binding properties of normal and cognitive performance-linked variant forms of KIBRA C2 domain

    Posner, M. G., Upadhyay, A., Ishima, R., Kalli, A. C., Harris, G., Kremerskothen, J., Sansom, M. S. P., Crennell, S. J. & Bagby, S., 15 Jun 2018, In : The Journal of biological chemistry. 293, 24, p. 9335-9344 10 p.

    Research output: Contribution to journalArticle

    Open Access
  • 2 Citations (Scopus)
    Open Access
    File
  • 18 Downloads (Pure)

    Structural insights of RmXyn10A - A prebiotic-producing GH10 xylanase with a non-conserved aglycone binding region

    Aronsson, A., Guler, F., Petoukhov, M., Crennell, S., Svergun, D., Linares-Pasten, J. & Nordbery Karlsson, E., 1 Feb 2018, In : Biochimica Et Biophysica Acta-Proteins and Proteomics. 1866, 2, p. 292-306 15 p.

    Research output: Contribution to journalArticle

    Open Access
  • 4 Citations (Scopus)

    The synthesis and kinetic evaluation of aryl α-aminophosphonates as novel inhibitors of T. cruzi trans-sialidase

    Chen, Z., Marce Villa, P., Resende, R., Alzari, P. M., Frasch, A. C., Van Den Elsen, J., Crennell, S. & Watts, A., 5 Oct 2018, In : European Journal of Medicinal Chemistry. 158, p. 25-33 9 p.

    Research output: Contribution to journalArticle

    Open Access
    File
  • 3 Citations (Scopus)
    12 Downloads (Pure)

    Thesis

    Carbohydrate-degrading enzymes from the thermophilic ethanologen Geobacillus thermoglucosidasius

    Author: Espina Silva, G., 20 May 2015

    Supervisor: Danson, M. (Supervisor) & Crennell, S. (Supervisor)

    Student thesis: Doctoral ThesisPhD

    File

    Identifying the wall-degrading enzymes responsible for microspore release from the pollen tetrad

    Author: Tratt, J., 12 Feb 2016

    Supervisor: Crennell, S. (Supervisor), Doughty, J. (Supervisor) & Scott, R. (Supervisor)

    Student thesis: Doctoral ThesisPhD

    File

    ProCoFFEE - Improved Protein Modelling Through Flexibility

    Author: Mcmanus, T., 20 Nov 2019

    Supervisor: Van Den Elsen, J. (Supervisor), Walker, A. (Supervisor) & Crennell, S. (Supervisor)

    Student thesis: Doctoral ThesisPhD

    File

    Structural and pharmacological study of Trypanosoma cruzi trans-sialidase

    Author: Chen, Z., 1 Oct 2017

    Supervisor: Crennell, S. (Supervisor), Van Den Elsen, J. (Supervisor) & Watts, A. (Supervisor)

    Student thesis: Doctoral ThesisPhD

    File