• 5 WEST 2.44

20002018
If you made any changes in Pure these will be visible here soon.

Personal profile

Research interests

The research in the laboratory is focused upon the investigation of membrane and cellular mechanisms regulating the arterial function in physiological conditions and in disease using contemporary laboratory methods which include small vessel myography, electrophysiological patch-clamp technique, molecular biological and imaging techniques.

Arteries determine blood pressure by changing their diameter which is controlled by the activity of ion channels. One group of ion channels ubiquitously present in smooth muscle cells (VSMCs) which compose the arterial wall, K+ channels are central for the regulation of resting membrane potential hence vascular tone. Among them voltage-gated K(Kv) channels are abundant in pulmonary arterial SMCs and contribute to an important physiological response to hypoxia termed hypoxic pulmonary vasoconstriction (HPV). We initially demonstrated a significant decrease in Kv channels expression (which would results in membrane depolarization and vasoconstriction) in chronic hypoxia in the rat (Smirnov et al., 1994), an animal model of pulmonary hypertension. Recently, we demonstrated that Kv channels in PASMCs are closely linked to the mitochondrial function via a novel mitochondrial-mediated Mg2+-dependent mechanism (Firth et al., 2008). This unique regulatory mechanism is due to an existence of sub-membrane population of mitochondria in PASMCs and is not present in systemic SMCs (Firth et al., 2009).

In collaboration with colleagues from the Department of Physics we developed a novel approach to investigate protein complexes on the cell surface using atomic and magnetic force microscopy. With this approach we successfully demonstrated distribution of individual endothelin receptor molecules on the surface of aortic SMCs (Moskalenko et al, 2010).

In collaboration with colleagues from the University of Calgary, we showed specific distribution along the renal vascular tree of another type of K channels, the inward rectifier (KIR), and demonstrated their functional importance in renal afferent arterioles which are central for blood pressure control (Chilton et al., 2011).

Keywords

  • QP Physiology
  • Vascular pharmacology
  • Electrophysiology
  • Ion channels
  • Smooth muscle
  • Cellular signalling
  • Arteries
  • Microcirculation

Fingerprint Dive into the research topics where Sergey Smirnov is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 2 Similar Profiles
Arteries Medicine & Life Sciences
Vasoconstriction Medicine & Life Sciences
Mesenteric Arteries Medicine & Life Sciences
Smooth Muscle Myocytes Medicine & Life Sciences
Pulmonary Artery Medicine & Life Sciences
Lung Medicine & Life Sciences
6-anilino-5,8-quinolinedione Medicine & Life Sciences
Mitochondria Medicine & Life Sciences

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Projects 2007 2018

Investigating the role of advanced glycation end product on vascular contractility

Smirnov, S., El-bassossy, H. M., Eid, B. G. & Balamash, K.

14/01/1714/01/18

Project: Other

Proteins
Endothelin A Receptors
Endothelin Receptors
Atomic Force Microscopy
Nanoparticles

Research Output 2000 2018

Enhanced calcium entry via activation of NOX/PKC underlies increased vasoconstriction induced by methylglyoxal

Eid, B. G., Sharib, A. A., El-bassossy, H. M., Balamash, K. & Smirnov, S., 2 Dec 2018, In : Biochemical and Biophysical Research Communications. 506, 4, p. 1013-1018 6 p.

Research output: Contribution to journalArticle

10 Citations (Scopus)
69 Downloads (Pure)

Sphingosylphosphorylcholine potentiates vasoreactivity and voltage-gated Ca2+ entry via NOX1 and reactive oxygen species

Shaifta, Y., Snetkov, V. A., Prieto-Lloret, J., Knock, G. A., Smirnov, S. V., Aaronson, P. I. & Ward, J. P. T., 1 Apr 2015, In : Cardiovascular Research. p. 121-130

Research output: Contribution to journalArticle

Open Access
File
Reactive Oxygen Species
Vasoconstriction
Mesenteric Arteries
6-anilino-5,8-quinolinedione
Arteries
19 Citations (Scopus)
68 Downloads (Pure)

TRPM4 inhibitor 9-phenanthrol activates endothelial cell intermediate conductance calcium-activated potassium channels in rat isolated mesenteric artery

Garland, C. J., Smirnov, S., Bagher, P., Lim, CS., Mitchell, R., Stanley, C., Pinkney, A. & Dora, K. A., Feb 2015, In : British Journal of Pharmacology. 172, 4, p. 1114-1123 10 p.

Research output: Contribution to journalArticle

File
Intermediate-Conductance Calcium-Activated Potassium Channels
Mesenteric Arteries
Endothelial Cells
Smooth Muscle
Vasoconstriction
16 Citations (Scopus)
67 Downloads (Pure)

Voltage-activated Ca2+ channels in rat renal afferent and efferent myocytes: no evidence for the T-type Ca2+ current

Smirnov, S. V., Loutzenhizer, K. & Loutzenhiser, R., 1 Feb 2013, In : Cardiovascular Research. 97, 2, p. 293-301 9 p.

Research output: Contribution to journalArticle

Open Access
File
Muscle Cells
Arterioles
Kidney
Tail
Arteries
7 Citations (Scopus)
66 Downloads (Pure)

β1-adrenoceptor stimulation suppresses endothelial IKCa-channel hyperpolarization and associated dilatation in resistance arteries

Iarova, P. L., Smirnov, S. V., Dora, K. A. & Garland, C. J., Jun 2013, In : British Journal of Pharmacology. 169, 4, p. 875-886 12 p.

Research output: Contribution to journalArticle

Open Access
File
Adrenergic Receptors
Dilatation
Arteries
Vasodilation
Endothelial Cells

Thesis

Investigation of the Mechanisms of Excitation and Contraction in Rat Pulmonary and Systemic Resistance Arteries

Author: Rahman, M., 1 Aug 2010

Supervisor: Smirnov, S. (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Mechanisms underlying the endothelium-dependent modulation of vascular tone

Author: Iarova, P., 1 Aug 2011

Supervisor: Dora, K. (Supervisor), Garland, C. (Supervisor), Smirnov, S. (Supervisor) & Jones, R. (External person) (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Molecular pharmacology of altered cardiopulmonary function in inflammation

Author: El-Awady, M., 1 Nov 2008

Supervisor: Watson, M. (Supervisor) & Smirnov, S. (Supervisor)

Student thesis: Doctoral ThesisPhD

File