James Doughty

Dr

  • 3 SOUTH 1.15

20032019
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Personal profile

Research interests

Current Research

My research interests are currently focused on molecular recognition events and signalling between the pollen and stigma in species belonging to the Brassicaceae (including Brassica oleracea and Arabidopsis thaliana).

Pollinations can be either compatible or incompatible and a molecular dialogue is established within minutes of the arrival of the pollen grain at the stigma surface that will lead either to acceptance or rejection of the pollen.

Brassica oleracea, in common with many flowering plant species, prevents self-fertilization by a mechanism termed self-incompatibility (SI). Simply, SI permits the recognition and rejection of 'self' pollen - an attribute that promotes genetic diversity and one that is held to have been crucial to the rapid adaptive radiation of flowering plants early in their evolutionary history. SI in Brassica is controlled by a single multiallelic locus, the S-locus. The female determinant is known to be a transmembrane receptor kinase, the SRK (S-receptor kinase), which is thought to be activated by binding the pollen-borne determinant of SI, SCR (for S cysteine-rich protein). Activation of SRK initiates a signalling cascade that ultimately leads to pollen rejection probably through denial of access to stigmatic water.

My research has established that SCR is but one member of a family of small cysteine-rich pollen coat proteins (the PCP-A class) that bind stigmatic proteins known to have roles in pollen-stigma interactions and recognition. These proteins tend to be gametophytically expressed (see figure) and are secreted from the pollen protoplast to ultimately end up on the surface of the grains. Studies are underway to further characterise this family of proteins and establish their functions during the pollination process.

Further, although the primary determinants of SI have been identified, gaining an understanding of the molecular basis of S-specific recognition and the precise mode of activation of the SI system is of particular interest in my lab.

Goals

To further our understanding of the molecular basis of pollen recognition and rejection both within and between species.

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Arabidopsis Medicine & Life Sciences
Pollen Medicine & Life Sciences
pollen Agriculture & Biology
Brassica Medicine & Life Sciences
cysteine Agriculture & Biology
Arabidopsis thaliana Agriculture & Biology
Protein S Medicine & Life Sciences
Cysteine Medicine & Life Sciences

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Research Output 2003 2019

A gamma-thionin protein from apple, MdD1, is required for defence against S-RNase-induced inhibition of pollen tube prior to self/non-self recognition

Gu, Z., Li, W., Doughty, J., Meng, D., Yang, Q., Yuan, H., Li, Y., Chen, Q., Yu, J., Liu, C. S. & Li, T., 1 Nov 2019, In : Plant Biotechnology Journal. 17, 11, p. 2184-2198 15 p.

Research output: Contribution to journalArticle

Open Access
17 Citations (Scopus)

A cationic diode based on asymmetric nafion film deposits

He, D., Aaronson, B. D. B., Madrid, E., Fan, L., Doughty, J., Mathwig, K., Bond, A. M., Mckeown, N. B. & Marken, F., 29 Mar 2017, In : ACS Applied Materials and Interfaces. 9, 12, p. 11272-11278

Research output: Contribution to journalArticle

Diodes
Deposits
Polyethylene Terephthalates
Charge carriers
Electric space charge
9 Citations (Scopus)
96 Downloads (Pure)

Ionic diodes based on regenerated α-cellulose films deposited asymmetrically onto a microhole

Aaronson, B. D. B., He, D., Madrid, E., Johns, M. A., Scott, J. L., Fan, L., Doughty, J., Kadowaki, M. A. S., Polikarpov, I., McKeown, N. B. & Marken, F., 23 Jan 2017, In : ChemistrySelect. 2, 3, p. 871-875 5 p.

Research output: Contribution to journalArticle

Open Access
File
Cellulose films
Cellulose
Diodes
Ionic Liquids
Polyethylene Terephthalates
5 Citations (Scopus)

RNAi inhibition of feruloyl CoA 6′-hydroxylase reduces scopoletin biosynthesis and post-harvest physiological deterioration in cassava (Manihot esculenta Crantz) storage roots

Liu, S., Zainuddin, I. M., Vanderschuren, H., Doughty, J. & Beeching, J. R., May 2017, In : Plant Molecular Biology. 94, 2, p. 185-195 11 p.

Research output: Contribution to journalArticle

Open Access
Scopoletin
scopoletin
Manihot
Manihot esculenta
RNA Interference

Genetic variation for triploid block in Arabidopsis thaliana

Wilkins, C-A., Bolbol, A., Qi, B., Doughty, J. & Scott, R., 18 Mar 2016.

Research output: Contribution to conferencePoster

Thesis

File

Diverse Roles of Protein S-acyl Transferases in Arabidopsis thaliana

Author: Li, Y., 26 Apr 2017

Supervisor: Scott, R. (Supervisor), Qi, B. (Supervisor) & Doughty, J. (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Identifying the wall-degrading enzymes responsible for microspore release from the pollen tetrad

Author: Tratt, J., 12 Feb 2016

Supervisor: Crennell, S. (Supervisor), Doughty, J. (Supervisor) & Scott, R. (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Maternal control of seed development mediated by the flavonoid biosynthesis pathway

Author: Aljabri, M., 22 Jun 2016

Supervisor: Doughty, J. (Supervisor) & Scott, R. (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Proteome and Transcriptional Analysis of Arabidopsis thaliana Sperm Cells

Author: Sriboonlert, A., 1 Sep 2008

Supervisor: Doughty, J. (Supervisor)

Student thesis: Doctoral ThesisPhD

File