Projects per year
Personal profile
Research interests
My main research interests are in the areas of insulin and exercise signalling, insulin resistance and glucose metabolism in physiological and pathophysiological conditions. My research brings new cellular and molecular approaches to study the pathophysiology of chronic metabolic conditions and helps understand the impact of nutritional and physiological changes on the development and treatments of insulin resistance, type 2 diabetes and metabolic syndrome.
For several years now, I have investigated the mechanism of glucose transporter (GLUT4) vesicle trafficking. Insulin signalling plays a pivotal role in this process. Therefore, mapping GLUT4 trafficking is critical to understanding the cellular defects in diabetes. The identification of the key molecules linking insulin signalling and GLUT4 trafficking is a major challenge in the GLUT4 research field. The reason this challenge remains is mainly due to the complexity of the insulin-signalling cascade. The next major challenge in this area is to identify the small GTPases of the Rab family of proteins responsible for insulin induced GLUT4 translocation. This is the aim of my current research grant from the MRC.
To achieve this aim I am using a cross-disciplinary approach, combining cellular and molecular studies with a human intervention study in order to translate the molecular findings into whole body physiological functions. We are using cellular models of adipose cells to investigate the molecular interactions between Rab3 and other proteins in the cell that act as links between insulin action at its receptor and the GLUT4 transporter. We will investigate how these interactions are affected by treatment with proinflammatory cytokines, including TNFα, or other molecules known to induce the state of insulin resistance. These experiments will allow us to understand the mechanisms of action of Rab3 in the context of the development of the insulin-resistant state. We will also investigate in humans, undergoing a diet and exercise intervention programme, whether Rab3 is affected in a manner that reveals underlying mechanisms involved in the control of insulin sensitivity in adipose tissues and skeletal muscles. The outcome from this project will have important implications for our understanding of the mechanisms of the development of peripheral tissues insulin resistance and for the development of new, targeted therapies for treatment of insulin resistant subjects and people with type 2 diabetes. This study will also be a springboard to inform further investigations into the development of insulin resistance and the cross-talk between chronic inflammation, obesity and the onset of type 2 diabetes.
Willing to supervise doctoral students
Identification of new biomarkers of insulin resistance and type 2 diabetes.
Development of human skeletal muscle satellite cell model for in vitro assessment of metabolic health.
Education/Academic qualification
Doctor of Philosophy, Université Grenoble Alpes
Award Date: 1 Jan 1996
Keywords
- QP Physiology
- Glucose metabolism
- glucose transporters
- Diabetes
- Insulin signalling
- Insulin resistance
- Rab GTPases
- membrane trafficking
- skeletal muscle
- adipocyes
- obesity
- Type 2 diabetes
- GLUT4
- Cell Biology
- Biochemistry
- Tissue culture
- exocytosis
- endocytosis
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Network
Projects
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Endosomal sorting complexes in insulin-stimulated GLUT4 recycling
1/09/19 → 31/12/20
Project: UK charity
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Imprinted genes defining a novel mammalian growth regulatory axis
1/06/19 → 31/05/22
Project: Research council
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Role of Rab3 in Peripheral Tissue Insulin Resistance
Koumanov, F., Betts, J., Gonzalez, J. & Thompson, D.
1/12/16 → 30/11/20
Project: Research council
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Elucidation of Final Stages in Coupling of Insulin Signalling to CLUT4 Translocation
1/07/12 → 30/06/15
Project: Research council
Research Output
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Ensilicated tetanus antigen retains immunogenicity: in vivo study and time-resolved SAXS characterization
Doekhie, A., Dattani, R., Chen, Y-C., Yang, Y., Smith, A., Silve, A. P., Koumanov, F., Wells, S., Edler, K., Marchbank, K. J., Van Den Elsen, J. & Sartbaeva, A., 1 Dec 2020, In: Scientific Reports. 10, 1, 9243.Research output: Contribution to journal › Article › peer-review
Open Access3 Citations (Scopus) -
Impact of pre-exercise feeding status on metabolic adaptations to endurance-type exercise training: Exercise-nutrient interactions and metabolic adaptation
Gonzalez, J., Edinburgh, R. & Koumanov, F., 29 Dec 2020, (Acceptance date) In: Journal of Physiology.Research output: Contribution to journal › Article › peer-review
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Lipid metabolism links nutrient-exercise timing to insulin sensitivity in men classified as overweight or obese. Breakfast, exercise and metabolic health
Edinburgh, R., Bradley, H., Abdullah, N-F., Robinson, S., Chrzanowski-Smith, O., Walhin, J-P., Joanisse, S., Manolopoulos, K., Philp, A., Hengist, A., Chabowski, A., Brodsky, F., Koumanov, F., Betts, J., Thompson, D., Wallis, G. & Gonzalez, J., 31 Mar 2020, In: Journal of Clinical Endocrinology & Metabolism. 105, 3, p. 660-676 17 p., dgz104.Research output: Contribution to journal › Article › peer-review
Open Access1 Citation (Scopus) -
Thermal resilience of ensilicated lysozyme via calorimetric and in vivo analysis
Doekhie, A., Slade, M., Cliff, L., Weaver, L., Castaing, R., Paulin, J., Chen, Y-C., Edler, K., Koumanov, F., Marchbank, K., Van Den Elsen, J. & Sartbaeva, A., 12 Aug 2020, In: RSC Advances. 10, 50, p. 29789-29796 8 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Fructose and metabolic health: governed by hepatic glycogen status?
Hengist, A., Koumanov, F. & Gonzalez, J., 15 Jul 2019, In: The Journal of Physiology. 597, 14, p. 3573-3585 13 p.Research output: Contribution to journal › Review article › peer-review
Open Access4 Citations (Scopus)
Datasets
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FEEDING INFLUENCES ADIPOSE TISSUE RESPONSES TO EXERCISE IN OVERWEIGHT MEN
Chen, Y. (Creator), Travers, R. (Researcher), Walhin, J. (Researcher), Gonzalez, J. (Researcher), Koumanov, F. (Researcher), Betts, J. (Researcher) & Thompson, D. (Project Leader), University of Bath, 2017
DOI: 10.15125/BATH-00361
Dataset
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Dataset for 'Pre-Exercise Breakfast Ingestion versus Extended Overnight Fasting Increases Postprandial Glucose Flux after Exercise in Healthy Men: Pre-exercise feeding and postprandial glucose flux'
Edinburgh, R. (Creator), Hengist, A. (Creator), Smith, H. (Creator), Travers, R. (Creator), Koumanov, F. (Creator), Betts, J. (Creator), Thompson, D. (Creator), Walhin, J. (Creator), Wallis, G. (Creator), Hamilton, D. L. (Creator), Stevenson, E. (Creator), Tipton, K. (Creator) & Gonzalez, J. (Creator), University of Bath, 27 Feb 2019
DOI: 10.15125/BATH-00608
Dataset
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Dataset for "Lipid metabolism links nutrient-exercise timing to insulin sensitivity in overweight men"
Edinburgh, R. (Creator), Bradley, H. (Creator), Abdullah, N. (Creator), Robinson, S. (Creator), Chrzanowski-Smith, O. (Creator), Walhin, J. (Creator), Joanisse, S. (Creator), Manolopoulos, K. (Creator), Philp, A. (Creator), Hengist, A. (Creator), Chabowski, A. (Creator), Brodsky, F. (Creator), Koumanov, F. (Creator), Betts, J. (Creator), Thompson, D. (Creator), Wallis, G. (Creator) & Gonzalez, J. (Creator), University of Bath, 19 Oct 2019
DOI: 10.15125/BATH-00672
Dataset
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Dataset for "Ensilicated tetanus antigen retains immunogenicity: in vivo study and time-resolved SAXS characterization"
Doekhie, A. (Creator), Sartbaeva, A. (Creator), Koumanov, F. (Creator), Van Den Elsen, J. (Creator), Edler, K. (Creator) & Wells, S. (Creator), University of Bath, 8 Jun 2020
DOI: 10.15125/BATH-00771
Dataset
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Dataset for "Thermal resilience of ensilicated lysozyme via calorimetric and in vivo analysis"
Doekhie, A. (Creator), Slade, M. (Creator), Castaing, R. (Creator), Edler, K. (Creator), Koumanov, F. (Creator), Van Den Elsen, J. (Creator), Marchbank, K. (Creator) & Sartbaeva, A. (Creator), University of Bath, 12 Aug 2020
DOI: 10.15125/BATH-00895
Dataset