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Research Output

2020

Pilot-scale production of xylo-oligosaccharides and fermentable sugars from Miscanthus using steam explosion pretreatment

Bhatia, R., Winters, A., Bryant, D. N., Bosch, M., Clifton-Brown, J., Leak, D. & Gallagher, J., 1 Jan 2020, In : Bioresource Technology. 296, 122285.

Research output: Contribution to journalArticle

Open Access
2019
Open Access

Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoA

Chacón, M. G., Kendrick, E. G. & Leak, D. J., 1 Jul 2019, In : PeerJ. 2019, 7, e6971.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)

Esterification of geraniol as a strategy for increasing product titre and specificity in engineered Escherichia coli

Chacón, M. G., Marriott, A., Kendrick, E. G., Styles, M. Q. & Leak, D. J., 8 Jun 2019, In : Microbial Cell Factories. 18, 1, p. 1-11 11 p., 105.

Research output: Contribution to journalArticle

Open Access
6 Citations (Scopus)

Heterologous Microcompartment Assembly in Bacillaceae: Establishing the Components Necessary for Scaffold Formation

Wade, Y., Daniel, R. A. & Leak, D. J., 19 Jul 2019, In : ACS Synthetic Biology. 8, 7, p. 1642-1654 13 p.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)

Higher value products from AD: a review on medium chain carboxylic acids from bio-waste

De Groof, V., Arnot, T., Leak, D., Coma, M. & Lanham, A., 24 Jan 2019.

Research output: Contribution to conferencePoster

Medium Chain Carboxylic Acids from Complex Organic Feedstocks by Mixed Culture Fermentation

De Groof, V., Coma, M., Arnot, T., Leak, D. J. & Lanham, A. B., 22 Jan 2019, In : Molecules. 24, 3, p. 1-32 32 p., 398.

Research output: Contribution to journalReview article

Open Access
10 Citations (Scopus)

Protein Manipulation

Leak, D. & Bartosiak-Jentys, J., 2019, Patent No. WO 2013/175190 (BR112014029129, EP2852663, US2015104850) , 20 May 2013, Priority date 22 May 2012, Priority No. 1209041.1

Research output: Patent

SYNTHESIS OF MONOTERPENOID ESTER COMPOUNDS

Chacon, M., Leak, D. & Styles, M., 16 May 2019, IPC No. C12P5/00; C12P7/02; C12P7/62;, Patent No. WO2019092388A1, 10 Nov 2017, Priority date 10 Nov 2017

Research output: Patent

2018

Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery

Cárdenas-Fernández, M., Hamley-Bennett, C., Leak, D. J. & Lye, G. J., 1 Dec 2018, In : Bioresource Technology. 269, p. 195-202 8 p.

Research output: Contribution to journalArticle

Open Access
3 Citations (Scopus)

Crystal structure of an inferred ancestral bacterial pyruvate decarboxylase

Buddrus, L., Andrews, E. S. V., Leak, D., Danson, M., Arcus, V. L. & Crennell, S., 1 Mar 2018, In : Acta Crystallographica Section F:Structural Biology Communications. 74, 3, p. 179-186 8 p.

Research output: Contribution to journalArticle

Open Access
2 Citations (Scopus)

Dielectric barrier discharge plasma microbubble reactor for pretreatment of lignocellulosic biomass

Wright, A., Bandulasena, H., Ibenegbu, C., Leak, D., Holmes, T., Zimmerman, W., Shaw, A. & Iza, F., 7 Sep 2018, In : AIChE Journal. 64, 11, p. 3803-3816 14 p.

Research output: Contribution to journalArticle

Open Access
8 Citations (Scopus)

Medium chain carboxylic acids from COD-rich food waste as sole substrate

De Groof, V., Arnot, T., Lanham, A., Coma, M. & Leak, D., 16 Jul 2018.

Research output: Contribution to conferenceAbstract

2017

An integrated biorefinery concept for conversion of sugar beet pulp into value-added chemicals and pharmaceutical intermediates

Cárdenas-Fernández, M., Bawn, M., Hamley-Bennett, C., Bharat, P. K. V., Subrizi, F., Suhaili, N., Ward, D. P., Bourdin, S., Dalby, P. A., Hailes, H. C., Hewitson, P., Ignatova, S., Kontoravdi, C., Leak, D. J., Shah, N., Sheppard, T. D., Ward, J. M. & Lye, G. J., 1 Sep 2017, In : Faraday Discussions. 202, p. 415-431

Research output: Contribution to journalArticle

Open Access
11 Citations (Scopus)

Centrifugal partition chromatography in a biorefinery context: Optimisation and scale-up of monosaccharide fractionation from hydrolysed sugar beet pulp

Ward, D. P., Hewitson, P., Cárdenas-Fernández, M., Hamley-Bennett, C., Díaz-Rodríguez, A., Douillet, N., Adams, J. P., Leak, D. J., Ignatova, S. & Lye, G. J., 12 May 2017, In : Journal of Chromatography A. 1497, p. 56-63 8 p.

Research output: Contribution to journalArticle

Open Access
10 Citations (Scopus)

Characterization of the first naturally thermostable terpene synthases and development of strategies to improve thermostability in this family of enzymes

Styles, M. Q., Nesbitt, E. A., Marr, S., Hutchby, M. & Leak, D. J., Jun 2017, In : FEBS Journal. 284, 11, p. 1700-1711

Research output: Contribution to journalArticle

Open Access
3 Citations (Scopus)

Development of an efficient technique for gene deletion and allelic exchange in Geobacillus spp.

Bacon, L. F., Hamley-Bennett, C., Danson, M. J. & Leak, D. J., 5 Apr 2017, In : Microbial Cell Factories. 16, 1, 8 p., 58.

Research output: Contribution to journalArticle

Open Access
3 Citations (Scopus)

Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris

Aw, R., Barton, G. R. & Leak, D. J., Jun 2017, In : Applied Microbiology and Biotechnology. 101, 12, p. 5045-5058

Research output: Contribution to journalArticle

Open Access
9 Citations (Scopus)

Novel thermostable antibiotic resistance enzymes from the Atlantis II Deep Red Sea brine pool

Elbehery, A. H. A., Leak, D. J. & Siam, R., Jan 2017, In : Microbial Biotechnology. 10, 1, p. 189-202

Research output: Contribution to journalArticle

Open Access
5 Citations (Scopus)

Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling

Tredwell, G. D., Aw, R., Edwards-Jones, B., Leak, D. J. & Bundy, J. G., Mar 2017, In : Journal of Industrial Microbiology and Biotechnology. 44, 3, p. 413-417 5 p.

Research output: Contribution to journalArticle

Open Access
8 Citations (Scopus)
2016

Crystal structure of pyruvate decarboxylase from Zymobacter palmae

Buddrus, L., Andrews, E. S. V., Leak, D. J., Danson, M. J., Arcus, V. L. & Crennell, S. J., 1 Sep 2016, In : Acta Crystallographica Section F:Structural Biology Communications. 72, 9, p. 700-706

Research output: Contribution to journalArticle

Open Access
File
5 Citations (Scopus)
84 Downloads (Pure)

Hybrid and mixed matrix membranes for separations from fermentations

Leak, D., Davey, C. & Patterson, D., 29 Feb 2016, In : Membranes. 6, 1, 17.

Research output: Contribution to journalArticle

Open Access
File
15 Citations (Scopus)
236 Downloads (Pure)

Nanofiltration and reverse osmosis membranes for purification and concentration of a 2,3-butanediol producing gas fermentation broth

Davey, C., Havill, A., Leak, D. & Patterson, D., 15 Nov 2016, In : Journal of Membrane Science. 518, p. 150-158 9 p.

Research output: Contribution to journalArticle

Open Access
File
5 Citations (Scopus)
174 Downloads (Pure)

Production of ethanol by thermophilic oligosaccharide utilising Geobacillus thermoglucosidasius TM242 using palm kernel cake as a renewable feedstock

Raita, M., Ibenegbu, C., Champreda, V. & Leak, D. J., 1 Dec 2016, In : Biomass and Bioenergy. 95, p. 45-54 10 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)
Open Access
File
18 Citations (Scopus)
271 Downloads (Pure)

The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering

Reeve, B., Martinez-Klimova, E., de Jonghe, J., Leak, D. J. & Ellis, T., 16 Dec 2016, In : ACS Synthetic Biology. 5, 12, p. 1342-1347 6 p.

Research output: Contribution to journalArticle

Open Access
19 Citations (Scopus)
115 Downloads (Pure)
2015

Chapter One - The genus Geobacillus and their biotechnological potential

Hussein, A. H., Lisowska, B. K. & Leak, D. J., 2015, In : Advances in Applied Microbiology. 92, p. 1-48 48 p.

Research output: Contribution to journalArticle

Open Access
File
36 Citations (Scopus)
251 Downloads (Pure)
11 Citations (Scopus)

PathwayBooster: a tool to support the curation of metabolic pathways

Liberal, R., Lisowska, B. K., Leak, D. J. & Pinney, J. W., 15 Mar 2015, In : BMC Bioinformatics. 16, 86.

Research output: Contribution to journalArticle

Open Access
File
2 Citations (Scopus)
130 Downloads (Pure)

Translational arrest due to cytoplasmic redox stress delays adaptation to growth on methanol and heterologous protein expression in a typical fed-batch culture of Pichia pastoris

Edwards-Jones, B., Aw, R., Barton, G. R., Tredwell, G. D., Bundy, J. G. & Leak, D. J., 18 Mar 2015, In : PLoS ONE. 10, 3, e0119637.

Research output: Contribution to journalArticle

Open Access
File
9 Citations (Scopus)
129 Downloads (Pure)
2014

Enzyme biotransformations and reactors

Emanuelsson, E. A. C. & Leak, D., 16 Dec 2014, Chemical Processes for a Sustainable Future. Letcher, T., Scott, J. & Patterson, D. (eds.). Royal Society of Chemistry

Research output: Chapter in Book/Report/Conference proceedingChapter

Global sensitivity analysis and meta-modeling of an ethanol production process

Todri, E., Amenaghawon, A. N., Del Val, I. J., Leak, D. J., Kontoravdi, C., Kucherenko, S. & Shah, N., 1 Jul 2014, In : Chemical Engineering Science. 114, p. 114-127

Research output: Contribution to journalArticle

20 Citations (Scopus)
2013

Application of 13C metabolic flux analysis to understanding the physiology of engineered Geobacillus Thermoglucosidasius

Ward, C. E. & Leak, D. J., 2013, p. 456-457. 2 p.

Research output: Contribution to conferencePaper

Modular system for assessment of glycosyl hydrolase secretion in Geobacillus thermoglucosidasius

Bartosiak-Jentys, J., Hussein, A. H., Lewis, C. J. & Leak, D. J., Jul 2013, In : Microbiology. 159, PART7, p. 1267-1275 9 p.

Research output: Contribution to journalArticle

28 Citations (Scopus)
2012
12 Citations (Scopus)

Aromatic Oxidations

Leak, D. J., Yin, Y., Zhang, J. J. & Zhou, N. Y., 20 Apr 2012, Enzyme Catalysis in Organic Synthesis, Third Edition. Wiley-VCH, Vol. 3. p. 1487-1533 47 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

OnpA, an unusual flavin-dependent monooxygenase containing a cytochrome b5 domain

Xiao, Y., Liu, T-T., Dai, H., Zhang, J-J., Liu, H., Tang, H., Leak, D. J. & Zhou, N-Y., Mar 2012, In : Journal of Bacteriology. 194, 6, p. 1342-1349 8 p.

Research output: Contribution to journalArticle

7 Citations (Scopus)
2011

Genetic tool development underpins recent advances in thermophilic whole-cell biocatalysts: Recent advances in thermophilic genetic systems

Taylor, M. P., Van Zyl, L., Tuffin, I. M., Leak, D. J. & Cowan, D. A., 2011, In : Microbial Biotechnology. 4, 4, p. 438-448

Research output: Contribution to journalArticle

22 Citations (Scopus)

Ionic liquid pretreatment of lignocellulosic biomass with ionic liquid–water mixtures

Brandt, A., Ray, M. J., To, T. Q., Leak, D. J., Murphy, R. J. & Welton, T., 2011, In : Green Chemistry. 13, 9, p. 2489-2499

Research output: Contribution to journalArticle

288 Citations (Scopus)

Ionic liquid pretreatment of lignocellulosic biomass with ionic liquid-water mixtures P51157WO

Brandt, A., Hallett, J. P., Leak, D. J., Murphy, R. J. & Welton, T., 2011

Research output: Patent

The development of metabolomic sampling procedures for Pichia pastoris, and baseline metabolome data

Tredwell, G. D., Edwards-Jones, B., Leak, D. J. & Bundy, J. G., 21 Jan 2011, In : PLoS ONE. 6, 1, 16 p., e16286.

Research output: Contribution to journalArticle

42 Citations (Scopus)
2010

Biobutanol: A better biofuel?

Crowhurst, N. A. & Leak, D. J., Feb 2010, In : International Sugar Journal. 112, 1334, p. 64-73 10 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)

Brown rot fungal early stage decay mechanism as a biological pretreatment for softwood biomass in biofuel production

Ray, M. J., Leak, D. J., Spanu, P. D. & Murphy, R. J., 2010, In : Biomass and Bioenergy. 34, 8, p. 1257-1262

Research output: Contribution to journalArticle

51 Citations (Scopus)

The effect of the ionic liquid anion in the pretreatment of pine wood chips

Brandt, A., Hallett, J. P., Leak, D. J., Murphy, R. J. & Welton, T., 1 Apr 2010, In : Green Chemistry. 12, 4, p. 672-679

Research output: Contribution to journalArticle

231 Citations (Scopus)

Venter creates synthetic life: Should we be scared?

Leak, D. J., 2010, In : Chemistry and Industry. 2010, 16, p. 16-17

Research output: Contribution to journalArticle

2009

Biocatalysts for selective introduction of oxygen

Leak, D. J., Sheldon, R. A., Woodley, J. M. & Adlercreutz, P., 2009, In : Biocatalysis and Biotransformation. 27, 1, p. 1-26

Research output: Contribution to journalArticle

64 Citations (Scopus)