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Research Output

2020

Peptide cargo tunes a network of correlated motions in human leucocyte antigens

Hopkins, J. R., Crean, R. M., Catici, D. A. M., Sewell, A. K., Arcus, V. L., Van der Kamp, M. W., Cole, D. K. & Pudney, C. R., 5 Mar 2020, In : FEBS Journal.

Research output: Contribution to journalArticle

Open Access

Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

Bowen, B. J., McGarrity, A. R., Szeto, J-Y. A., Pudney, C. R. & Jones, D. D., 21 Apr 2020, In : Chemical communications (Cambridge, England). 56, 31, p. 4308-4311 4 p.

Research output: Contribution to journalArticle

2019
Open Access
1 Citation (Scopus)

Excitation-energy-dependent molecular beacon detects early stage neurotoxic aβ aggregates in the presence of cortical neurons

Gulácsy, C. E., Meade, R., Catici, D. A. M., Soeller, C., Pantos, G. D., Jones, D. D., Alibhai, D., Jepson, M., Valev, V. K., Mason, J. M., Williams, R. J. & Pudney, C. R., 20 Mar 2019, In : ACS Chemical Neuroscience. 10, 3, p. 1240-1250 11 p.

Research output: Contribution to journalArticle

Open Access
File
1 Citation (Scopus)
40 Downloads (Pure)

Exposing the Interplay Between Enzyme Turnover, Protein Dynamics and the Membrane Environment in Monoamine Oxidase B

Jones, H. B. L., Crean, R. M., Mullen, A., Kendrick, E., Bull, S. D., Wells, S. A., Carbery, D. R., MacMillan, F., van der Kamp, M. W. & Pudney, C. R., 7 May 2019, In : Biochemistry. 58, 18, p. 2362-2372 11 p.

Research output: Contribution to journalArticle

Open Access
File
29 Downloads (Pure)

Synthetic Cannabinoid Receptor Agonists Detection using Fluorescence Spectral Fingerprinting

May, B., Naqi, H., Tipping, M., Scott, J., Husbands, S., Blagbrough, I. & Pudney, C., 15 Oct 2019, In : Analytical Chemistry. 91, 20, p. 12971-12979 9 p.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)
2018

Uncovering the relationship between the change in heat capacity for enzyme catalysis and vibrational frequency through isotope effect studies

Jones, H. B. L., Crean, R. M., Matthews, C., Troya, A. B., Danson, M. J., Bull, S. D., Arcus, V. L., Van Der Kamp, M. W. & Pudney, C. R., 1 Jun 2018, In : ACS Catalysis. 8, 6, p. 5340-5349 10 p.

Research output: Contribution to journalArticle

Open Access
File
4 Citations (Scopus)
50 Downloads (Pure)
2017

A complete thermodynamic analysis of enzyme turnover links the free energy landscape to enzyme catalysis

Jones, H. B. L., Wells, S. A., Prentice, E. J., Kwok, A., Liang, L. L., Arcus, V. L. & Pudney, C. R., 1 Sep 2017, In : FEBS Journal. 284, 17, p. 2829-2842 14 p.

Research output: Contribution to journalArticle

8 Citations (Scopus)

PROTEIN STRUCTURE ANALYSIS BASED ON RED-EDGE EXCITATION SHIFT (REES) SPECTROSCOPY

Pudney, C., 21 Sep 2017, IPC No. G01N21/64;, Patent No. WO 2017/158371 (EP3430382 , US2019072491), 17 Mar 2017, Priority date 18 Mar 2016, Priority No. 1604640.1

Research output: Patent

Steady-State Kinetics of α-Synuclein Ferrireductase Activity Identifies the Catalytically Competent Species

McDowall, J. S., Ntai, I., Hake, J., Whitley, P. R., Mason, J. M., Pudney, C. R. & Brown, D. R., 16 May 2017, In : Biochemistry. 56, 19, p. 2497–2505 9 p.

Research output: Contribution to journalArticle

Open Access
File
11 Citations (Scopus)
76 Downloads (Pure)
2016

Measuring protein flexibility and conformational state

Pudney, C., 2016, Patent No. 1604640.1, Priority date 18 Mar 2016

Research output: Patent

Modelling flavoenzymatic charge transfer events: development of catalytic indole deuteration strategies

Murray, A. T., Challinor, J. D., Gulácsy, C. E., Lujan, C., Hatcher, L. E., Pudney, C. R., Raithby, P. R., John, M. P. & Carbery, D. R., 21 Apr 2016, In : Organic and Biomolecular Chemistry. 14, 15, p. 3787-3792

Research output: Contribution to journalArticle

1 Citation (Scopus)

On the temperature dependence of enzyme-catalyzed rates

Arcus, V. L., Prentice, E. J., Hobbs, J. K., Mulholland, A. J., van der Kamp, M. W., Pudney, C. R., Parker, E. J. & Schipper, L. A., 29 Mar 2016, In : Biochemistry. 55, 12, p. 1681-1688

Research output: Contribution to journalArticle

Open Access
File
63 Citations (Scopus)
179 Downloads (Pure)

The red edge excitation shift phenomenon can be used to unmask protein structural ensembles: implications for NEMO-ubiquitin interactions

Catici, D. A. M., Amos, H. E., Yang, Y., van den Elsen, J. M. H. & Pudney, C. R., 20 Jun 2016, In : FEBS Journal. 283, 12, p. 2272-2284

Research output: Contribution to journalArticle

Open Access
File
10 Citations (Scopus)
144 Downloads (Pure)
2015

Change in heat capacity accurately predicts vibrational coupling in enzyme catalyzed reactions

Arcus, V. L. & Pudney, C. R., 4 Aug 2015, In : FEBS Letters. 589, 17, p. 2200-2206 7 p.

Research output: Contribution to journalArticle

10 Citations (Scopus)

Poly-ubiquitin drives the molecular interactions of NF-κB essential modulator (NEMO) by allosteric regulation

Catici, D. A. M., Horne, J. E., Cooper, G. E. & Pudney, C. R., 29 May 2015, In : Journal of Biological Chemistry. 290, 22, p. 14130-14139 10 p.

Research output: Contribution to journalArticle

8 Citations (Scopus)
2014

Practical aspects on the use of kinetic isotope effects as probes of flavoprotein enzyme mechanisms

Pudney, C. R., Hay, S. & Scrutton, N. S., 2014, Flavins and Flavoproteins. Weber, S. & Schleicher, E. (eds.). New York: Humana Press, p. 161-175 15 p. (Methods in Molecular Biology; vol. 1146).

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)
2013

Enzymatic single-molecule kinetic isotope effects

Pudney, C. R., Lane, R. S. K., Fielding, A. J., Maggenis, S. W., Hay, S. & Scrutton, N. S., 13 Mar 2013, In : Journal of the American Chemical Society. 135, 10, p. 3855-3864 10 p.

Research output: Contribution to journalArticle

Open Access
File
15 Citations (Scopus)
232 Downloads (Pure)

Excited state dynamics can be used to probe donor-acceptor distances for H-tunneling reactions catalysed by flavoproteins

Hardman, S., Pudney, C. R., Hay, S. & Scrutton, N. S., 3 Dec 2013, In : Biophysical Journal. 105, 11, p. 2549-2558 10 p.

Research output: Contribution to journalArticle

8 Citations (Scopus)

Fast protein motions are coupled to enzyme H-transfer reactions

Pudney, C., Guerriero, A., Baxter, N. J., Johannissen, L. O., Waltho, J. P., Hay, S. & Scrutton, N. S., Feb 2013, In : Journal of the American Chemical Society. 135, 7, p. 2512-2517 3 p.

Research output: Contribution to journalArticle

Open Access
File
68 Citations (Scopus)
136 Downloads (Pure)
2012

Gating mechanisms for biological electron transfer: Integrating structure with biophysics reveals the nature of redox control in cytochrome P450 reductase and copper-dependent nitrite reductase

Leferink, N. G. H., Pudney, C., Brenner, S., Heyes, D. J., Eady, R. R., Hasnain, S. S., Hay, S., Rigby, S. E. J. & Scrutton, N. S., 9 Mar 2012, In : FEBS Letters. 586, 5, p. 578-584

Research output: Contribution to journalArticle

22 Citations (Scopus)

Kinetic and spectroscopic probes of motions and catalysis in the cytochrome P450 Reductase family of enzymes

Pudney, C., Heyes, D. J., Khara, B., Hay, S., Rigby, S. E. J. & Scrutton, N. S., May 2012, In : FEBS Journal. 279, 9, p. 1534-1544

Research output: Contribution to journalArticle

20 Citations (Scopus)
2011

Coupled motions direct electrons along human microsomal P450 chains

Pudney, C. R., Khara, B., Johannissen, L. O. & Scrutton, N. S., 20 Dec 2011, In : PLoS Biology. 9, 12, 10 p., e1001222.

Research output: Contribution to journalArticle

42 Citations (Scopus)
2010

Biocatalysis with thermostable enzymes: Structure and properties of a thermophilic “ene”-reductase related to Old Yellow Enzyme

Adalbjornson, B., Fryszkowska, A., Toogood, H., Pudney, C., Jowitt, T. A., Leys, D. & Scrutton, N. S., 25 Jan 2010, In : ChemBiochem. 11, 2, p. 197-207

Research output: Contribution to journalArticle

83 Citations (Scopus)

Direct analysis of donor-acceptor distance and relationship to isotope effects and the force constant for barrier compression in enzymatic H-tunneling reactions

Pudney, C., Johannissen, L. O., Sutcliffe, M. J., Hay, S. & Scrutton, N. S., 2010, In : Journal of the American Chemical Society. 132, 32, p. 11329-11335

Research output: Contribution to journalArticle

63 Citations (Scopus)

Probing active site geometry using high pressure and secondary isotope effects in an enzyme-catalysed ‘deep’ H-tunneling reaction

Hay, S., Pudney, C., Sutcliffe, M. J. & Scrutton, N. S., Jul 2010, In : Journal of Physical Organic Chemistry. 23, 7, p. 696-701

Research output: Contribution to journalArticle

12 Citations (Scopus)
2009

Barrier compression enhances an enzymatic hydrogen-transfer reaction

Hay, S., Pudney, C., McGrory, T. A., Pang, J., Sutcliffe, M. J. & Scrutton, N. S., 9 Feb 2009, In : Angewandte Chemie-International Edition. 121, 8, p. 1480-1483

Research output: Contribution to journalArticle

46 Citations (Scopus)
20 Citations (Scopus)

Evidence to support the hypothesis that promoting vibrations enhance the rate of an enzyme catalyzed H-tunneling reaction

Pudney, C., Hay, S., Levy, C., Pang, J., Sutcliffe, M. J., Leys, D. & Scrutton, N. S., 2009, In : Journal of the American Chemical Society. 131, 47, p. 17072-17073

Research output: Contribution to journalArticle

63 Citations (Scopus)

Parallel pathways and free-energy landscapes for enzymatic hydride transfer probed by hydrostatic pressure

Pudney, C., McGrory, T., Lafite, P., Pang, J., Hay, S., Leys, D., Sutcliffe, M. J. & Scrutton, N. S., 25 May 2009, In : ChemBiochem. 10, 8, p. 1379-1384

Research output: Contribution to journalArticle

19 Citations (Scopus)

Structural and mechanistic aspects of flavoproteins: Probes of hydrogen tunneling

Hay, S., Pudney, C. & Scrutton, N. S., Aug 2009, In : FEBS Journal. 276, 15, p. 3930-3941

Research output: Contribution to journalArticle

24 Citations (Scopus)
2008

Are environmentally coupled enzymatic hydrogen tunneling reactions influenced by changes in solution viscosity?

Hay, S., Pudney, C., Sutcliffe, M. J. & Scrutton, N. S., 4 Jan 2008, In : Angewandte Chemie-International Edition. 47, 3, p. 537-540

Research output: Contribution to journalArticle

22 Citations (Scopus)

Atomistic insight into the origin of the temperature-dependence of kinetic isotope effects and H-tunnelling in enzyme systems is revealed through combined experimental studies and biomolecular simulation

Hay, S., Pudney, C., Hothi, P., Johannissen, L. O., Masgrau, L., Pang, J., Leys, D., Sutcliffe, M. J. & Scrutton, N. S., 2008, In : Biochemical Society Transactions. 36, p. 16-21

Research output: Contribution to journalArticle

18 Citations (Scopus)

Correction of pre-steady-state KIEs for isotopic impurities and the consequences of kinetic isotope fractionation

Hay, S., Pudney, C., Hothi, P. & Scrutton, N. S., 2008, In : The Journal of Physical Chemistry A. 112, 50, p. 13109-13115

Research output: Contribution to journalArticle

9 Citations (Scopus)

Solvent as a probe of active site motion and chemistry during the hydrogen tunnelling reaction in morphinone reductase

Hay, S., Pudney, C., Sutcliffe, M. J. & Scrutton, N. S., 15 Sep 2008, In : ChemPhysChem. 9, 13, p. 1875-1881

Research output: Contribution to journalArticle

14 Citations (Scopus)
2007

Mutagenesis of morphinone reductase induces multiple reactive configurations and identifies potential ambiguity in kinetic analysis of enzyme tunneling mechanisms

Pudney, C., Hay, S., Pang, J., Costello, C., Leys, D., Sutcliffe, M. J. & Scrutton, N. S., 2007, In : Journal of the American Chemical Society. 129, 45, p. 13949-13956

Research output: Contribution to journalArticle

40 Citations (Scopus)
2006
58 Citations (Scopus)