If you made any changes in Pure these will be visible here soon.

Personal profile

Research interests

The relationship between the structure of a protein and its function is vital to understanding how molecules give rise to biological effects. However, there is an emerging realisation that it is the flexibility and dynamics of proteins that in many cases drives their functional activity. Understanding this relationship is the next step in our understanding of basic life processes.

The scientific approach we take is to apply a broad range of high-level biophysical techniques, giving detailed quantitative information that provides novel insight. The lab has three major research strands:

New biotechnology

We are developing new experimental approaches to rapidly test proteins for their native function, based on accurate detection of their dynamics and flexibility, what we term the 'dynamic profile'. This technology has major industrial applications but also potential for use in a clinical setting.

Enzyme dynamics

One of the big questions in enzymology is the role of the motion of the protein, the enzyme dynamics. In particular, what is the role of dynamics in determining enzyme specificity, mechanism and activity. These are challenging questions, but answering them will contribute to enzyme biotechnology, for example improving enzyme (re)design.

Disordered protein biophysics

There is a very deep rooted notion that proteins must adopt very fixed structures and this fixed structure defines the functional abilities of the protein. However, a very high percentage of proteins contain large regions that are structurally disordered, lacking a defined structure as we currently define it. We are probing the functional role of structural disorder and how this feeds into molecular signalling networks.

Willing to supervise doctoral students

We have projects available in biopharmaceutical technology and biocatalysis.

Keywords

  • Enzyme
  • Catalysis
  • Fluorescence Spectroscopy
  • Biophysics
  • Single molecule
  • Biotechnology

Fingerprint Dive into the research topics where Christopher Pudney is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Projects

Research Output

Peptide cargo tunes a network of correlated motions in human leucocyte antigens

Hopkins, J. R., Crean, R. M., Catici, D. A. M., Sewell, A. K., Arcus, V. L., Van der Kamp, M. W., Cole, D. K. & Pudney, C. R., 5 Mar 2020, In : FEBS Journal.

Research output: Contribution to journalArticle

Open Access

Specificity of bispecific T cell receptors and antibodies targeting peptide-HLA

Holland, C. J., Crean, R. M., Pentier, J. M., de Wet, B., Lloyd, A., Srikannathasan, V., Lissin, N., Lloyd, K. A., Blicher, T. H., Conroy, P. J., Hock, M., Pengelly, R. J., Spinner, T. E., Cameron, B., Potter, E. A., Jeyanthan, A., Molloy, P. E., Sami, M., Aleksic, M., Liddy, N. & 9 others, Robinson, R. A., Harper, S., Lepore, M., Pudney, C. R., van der Kamp, M. W., Rizkallah, P. J., Jakobsen, B. K., Vuidepot, A. & Cole, D. K., 1 May 2020, In : Journal of Clinical Investigation. 130, 5, p. 2673-2688 16 p.

Research output: Contribution to journalArticle

Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

Bowen, B. J., McGarrity, A. R., Szeto, J-Y. A., Pudney, C. R. & Jones, D. D., 21 Apr 2020, In : Chemical communications (Cambridge, England). 56, 31, p. 4308-4311 4 p.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)

Excitation-energy-dependent molecular beacon detects early stage neurotoxic aβ aggregates in the presence of cortical neurons

Gulácsy, C. E., Meade, R., Catici, D. A. M., Soeller, C., Pantos, G. D., Jones, D. D., Alibhai, D., Jepson, M., Valev, V. K., Mason, J. M., Williams, R. J. & Pudney, C. R., 20 Mar 2019, In : ACS Chemical Neuroscience. 10, 3, p. 1240-1250 11 p.

Research output: Contribution to journalArticle

Open Access
File
1 Citation (Scopus)
40 Downloads (Pure)

Thesis

A Biophysical Approach to Understanding the Temperature-dependence of Enzyme Catalysis

Author: Jones, H., 2019

Supervisor: Leak, D. (Supervisor) & Pudney, C. (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Biophysical factors governing the structure, function, and dynamics of the NF-κB essential modulator protein (NEMO)

Author: Catici, A. M. D., 19 Jun 2019

Supervisor: Leak, D. (Supervisor) & Pudney, C. (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Exploring the substrate specificity of the antimicrobial peptide transporter BceAB of Bacillus subtilis

Author: Kobras, C. M., 29 May 2019

Supervisor: Pudney, C. (Supervisor) & Gebhard, S. (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Helical constraints and in silico library screening to develop novel antagonists of oncogenic Activator Protein-1

Author: Lathbridge, A., 19 Feb 2020

Supervisor: Van Den Elsen, J. (Supervisor), Pudney, C. (Supervisor) & Mason, J. (Supervisor)

Student thesis: Doctoral ThesisPhD

File

Photochemical and Spectroscopic Studies of Ketoaziridines

Author: Gulácsy, C., 8 Jun 2016

Supervisor: Carbery, D. (Supervisor) & Pudney, C. (Supervisor)

Student thesis: Doctoral ThesisPhD

File