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Research interests

Our research focuses on understanding the mechanisms of inflammation and inflammatory disease.

Cell biology of immune cells

My research group is interested in innate immunity including macrophage and microglia inflammatory responses. In particular, we have an interest in molecular signalling pathways, including reactive oxygen species and the role of hypoxic regulation, underlying the activation of the NLRP3 inflammasome and the production of the inflammatory cytokine interleukin 1 (IL-1Beta).

The production of bioactive IL-1Beta is a tightly controlled multi-step process: first IL-1Beta is produced as an inactivate precursor 31 kD pro-IL-1Beta, this is cleaved to produce bioactive 17 kD IL-1Beta and then finally the bioactive IL-1Beta. The processing and cleavage of the IL-1Beta precursor is achieved by caspase-1 activated within a multi-protein complex termed the NLRP3 inflammasome. We have specific interest in the pathways leading to activation of the NLRP3 inflammasome activation in macrophages and microglia.

Sites of inflammation exist under hypoxic conditions where changes in oxygen levels alter the phenotype of cells and the inflammatory response.  The research group is interested in understanding how hypoxia regulates inflammatory responses, in particular inflammasome responses, compared to standard tissue culture conditions.  The Advanced Hypoxic facility was funded by the BBSRC ALERT14 equipment call.

Our research is funded by the EPSRC, BBSRC and the University of Bath.

Fingerprint Dive into the research topics where Amanda Mackenzie is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

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Inflammasomes Medicine & Life Sciences
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Glycosylation Chemical Compounds
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Caspase 1 Medicine & Life Sciences
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Projects 2005 2020

MRC P2D - Showcase Event

Mackenzie, A.



Project: Research council

Electron Spin Resonance Spectroscopy

The P2X7 Interactome

Mackenzie, A.



Project: Research council

Adenosine Triphosphate
Purinergic P2X7 Receptors

Redox Signalling

Mackenzie, A.

The Royal Society


Project: Research council

Research Output 2001 2018

  • 18 Article
  • 5 Patent
  • 4 Meeting abstract
  • 1 Conference contribution
14 Citations (Scopus)
15 Downloads (Pure)

De novo mutations in GRIN1 cause extensive bilateral polymicrogyria

Fry, A., Fawcett, K., Zelnik, N., Yuan, H., Thomposon, B., Shemer-Meiri, L., cushion, T., Mugalaasi, H., Sims, D., Stoodley, N., Chung, S-K., Rees, M. I., Patel, C. V., Brueton, L. A., Layet, V., Giuliano, F., Kerr, M. P., Banne, E., Meiner, V., Lerman-Sagie, T. & 21 others, Helbig, K. L., Kofman, L. H., Knight, K. M., Chen, W., Kannan, V., Hu, C., Kusumoto, H., Zhang, J., Swanger, S. A., Shaulsky, G. H., Mirzaa, G. M., Muir, A. M., Mefford, H. C., Dobyns, W. B., Mackenzie, A., Mullins, J. G. L., Lemke, J. R., Bahi-Buisson, N., Traynelis, S. F., Iago, H. F. & Pilz, D. T., 31 Mar 2018, In : Brain : A Journal of Neurology. 141, 3, p. 698-712 15 p.

Research output: Contribution to journalArticle

Open Access
N-Methyl-D-Aspartate Receptors
Missense Mutation
12 Citations (Scopus)
30 Downloads (Pure)
Open Access
Peroxynitrous Acid
Reactive Oxygen Species
Oxidative stress
2 Citations (Scopus)
231 Downloads (Pure)
Open Access
Illness Behavior
108 Downloads (Pure)

Editorial: roles of ion channels in immune cells

Stokes, L., MacKenzie, A. B. & Sluyter, R., 15 Feb 2016, In : Frontiers in Immunology. 7, 48.

Research output: Contribution to journalArticle

Open Access

The profile of P2X7 receptor expression and properties in polarized primary mouse microglia cultures

Jackson, M., Wickens, R., Bailey, S. & Mackenzie, A., 2016, In : Journal of Psychopharmacology . 30, Abstract Supplement to volume 8, p. A98

Research output: Contribution to journalMeeting abstract

Purinergic P2X7 Receptors
Adenosine Triphosphate


Amyloid peptides and the vascular system : the link between cerebrovascular angiopathy and Alzheimer's disease

Author: Alsheikh Abubaker, A., Dec 2019

Supervisor: Eggleston, I. (Supervisor), Mackenzie, A. (Supervisor) & Pula, G. (Supervisor)

Student thesis: Doctoral ThesisPhD


Molecular mechanisms of YAP signalling in the context of cancer

Author: Xie, B., 13 Feb 2019

Supervisor: De Bank, P. (Supervisor), Mackenzie, A. (Supervisor), Ward, S. (Supervisor) & Mrsny, R. (Supervisor)

Student thesis: Doctoral ThesisPhD


Targeted Fluorescent Probes for Diagnostic Applications

Author: Weber, M., 15 Jan 2020

Supervisor: James, T. (Supervisor), Bull, S. (Supervisor) & Mackenzie, A. (Supervisor)

Student thesis: Doctoral ThesisPhD

The Development of Biological Therapeutics

Author: Ali-Hassan, S., 31 Dec 2013

Supervisor: Mackenzie, A. (Supervisor) & Watts, A. (Supervisor)

Student thesis: Doctoral ThesisMPhil


The Functional Expression of N-Methyl-D-Aspartate Glutamate-Type Receptors by Megakaryocytes and Platelets

Author: Hobbs, C., 1 May 2010

Supervisor: Mackenzie, A. (Supervisor)

Student thesis: Doctoral ThesisPhD