Amanda Mackenzie

Senior Lecturer, Department of Pharmacy & Pharmacology
Centre for Therapeutic Innovation

https://orcid.org/0000-0002-9935-4188

Phone+44 (0) 1225 385796
EmailA.Mackenzie@bath.ac.uk

7 WEST 3.10

Accepting PhD Students

Research output per year

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Personal profile

Research interests

Our research focuses on understanding the mechanisms of inflammation and inflammatory disease.

Cell biology of immune cells

My research group is interested in innate immunity including macrophage and microglia inflammatory responses. In particular, we have an interest in molecular signalling pathways, including reactive oxygen species and the role of hypoxic regulation, underlying the activation of the NLRP3 inflammasome and the production of the inflammatory cytokine interleukin 1 (IL-1Beta).

The production of bioactive IL-1Beta is a tightly controlled multi-step process: first IL-1Beta is produced as an inactivate precursor 31 kD pro-IL-1Beta, this is cleaved to produce bioactive 17 kD IL-1Beta and then finally the bioactive IL-1Beta. The processing and cleavage of the IL-1Beta precursor is achieved by caspase-1 activated within a multi-protein complex termed the NLRP3 inflammasome. We have specific interest in the pathways leading to activation of the NLRP3 inflammasome activation in macrophages and microglia.

Sites of inflammation exist under hypoxic conditions where changes in oxygen levels alter the phenotype of cells and the inflammatory response. The research group is interested in understanding how hypoxia regulates inflammatory responses, in particular inflammasome responses, compared to standard tissue culture conditions. The Advanced Hypoxic facility was funded by the BBSRC ALERT14 equipment call.

Our research is funded by the EPSRC, BBSRC and the University of Bath.

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Projects

1 Active
8 Finished

Targeting Molecular Pathways Leading to Retinal Dysfunction in Age Related Macular Degeneration

Mackenzie, A. & Watson, M.

Dunhill Medical Trust

7/01/19 → 6/12/20

Project: UK charity

MRC P2D - Showcase Event

Mackenzie, A.

MRC

31/03/18 → 31/10/18

Project: Research council

A Facility for Advanced Imaging and Analysis under Hypoxic Conditions for Bath Scientists and GW4 Partners

Mackenzie, A., Lindsay, M., Pourzand, C., Pula, G., Ward, S. & Williams, R.

BBSRC

1/01/15 → 31/12/15

Project: Research council

The P2X7 Interactome

Mackenzie, A.

BBSRC

17/01/13 → 16/01/16

Project: Research council

Redox Signalling

Mackenzie, A.

The Royal Society

29/06/08 → 2/07/08

Project: Research council

Research Output

19 Article
5 Patent
4 Meeting abstract
1 Conference contribution

Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes

Weber, M., Yamada, N., Tian, X., Bull, S. D., Minoshima, M., Kikuchi, K., Mackenzie, A. B. & James, T. D., 20 Feb 2020, In : Frontiers in Chemistry. 8, 39.

Research output: Contribution to journal › Article

Open Access

De novo mutations in GRIN1 cause extensive bilateral polymicrogyria

Fry, A., Fawcett, K., Zelnik, N., Yuan, H., Thomposon, B., Shemer-Meiri, L., cushion, T., Mugalaasi, H., Sims, D., Stoodley, N., Chung, S-K., Rees, M. I., Patel, C. V., Brueton, L. A., Layet, V., Giuliano, F., Kerr, M. P., Banne, E., Meiner, V., Lerman-Sagie, T. & 21 others, Helbig, K. L., Kofman, L. H., Knight, K. M., Chen, W., Kannan, V., Hu, C., Kusumoto, H., Zhang, J., Swanger, S. A., Shaulsky, G. H., Mirzaa, G. M., Muir, A. M., Mefford, H. C., Dobyns, W. B., Mackenzie, A., Mullins, J. G. L., Lemke, J. R., Bahi-Buisson, N., Traynelis, S. F., Iago, H. F. & Pilz, D. T., 31 Mar 2018, In : Brain : A Journal of Neurology. 141, 3, p. 698-712 15 p.

Research output: Contribution to journal › Article

Open Access