Projects per year
Personal profile
Research interests
Our research focuses on understanding the mechanisms of inflammation and inflammatory disease.
Cell biology of immune cells
My research group is interested in innate immunity including macrophage and microglia inflammatory responses. In particular, we have an interest in molecular signalling pathways, including reactive oxygen species and the role of hypoxic regulation, underlying the activation of the NLRP3 inflammasome and the production of the inflammatory cytokine interleukin 1 (IL-1Beta).
The production of bioactive IL-1Beta is a tightly controlled multi-step process: first IL-1Beta is produced as an inactivate precursor 31 kD pro-IL-1Beta, this is cleaved to produce bioactive 17 kD IL-1Beta and then finally the bioactive IL-1Beta. The processing and cleavage of the IL-1Beta precursor is achieved by caspase-1 activated within a multi-protein complex termed the NLRP3 inflammasome. We have specific interest in the pathways leading to activation of the NLRP3 inflammasome activation in macrophages and microglia.
Sites of inflammation exist under hypoxic conditions where changes in oxygen levels alter the phenotype of cells and the inflammatory response. The research group is interested in understanding how hypoxia regulates inflammatory responses, in particular inflammasome responses, compared to standard tissue culture conditions. The Advanced Hypoxic facility was funded by the BBSRC ALERT14 equipment call.
Our research is funded by the EPSRC, BBSRC and the University of Bath.
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Projects 2005 2020
Targeting Molecular Pathways Leading to Retinal Dysfunction in Age Related Macular Degeneration
7/01/19 → 6/07/20
Project: UK charity
A Facility for Advanced Imaging and Analysis under Hypoxic Conditions for Bath Scientists and GW4 Partners
Mackenzie, A., Lindsay, M., Pourzand, C., Pula, G., Ward, S. & Williams, R.
1/01/15 → 31/12/15
Project: Research council
Research Output 2001 2018
De novo mutations in GRIN1 cause extensive bilateral polymicrogyria
Fry, A., Fawcett, K., Zelnik, N., Yuan, H., Thomposon, B., Shemer-Meiri, L., cushion, T., Mugalaasi, H., Sims, D., Stoodley, N., Chung, S-K., Rees, M. I., Patel, C. V., Brueton, L. A., Layet, V., Giuliano, F., Kerr, M. P., Banne, E., Meiner, V., Lerman-Sagie, T. & 21 others, , 31 Mar 2018, In : Brain : A Journal of Neurology. 141, 3, p. 698-712 15 p.Research output: Contribution to journal › Article
Fluorescence based Tool to Detect Endogenous Peroxynitrite in M1-Polarized Murine J774.2 Macrophages
Weber, M., Mackenzie, A. B., Bull, S. D. & James, T. D., 4 Sep 2018, In : Analytical Chemistry. 90, 17, p. 10621–10627 7 p.Research output: Contribution to journal › Article
Repeated daily administration of increasing doses of lipopolysaccharide provides a model of sustained inflammation-induced depressive-like behaviour in mice that is independent of the NLRP3 inflammasome
Wickens, R. A., Ver Donck, L., Mackenzie, A. B. & Bailey, S. J., 15 Oct 2018, In : Behavioural Brain Research. 352, p. 99-108 10 p.Research output: Contribution to journal › Article
Editorial: roles of ion channels in immune cells
Stokes, L., MacKenzie, A. B. & Sluyter, R., 15 Feb 2016, In : Frontiers in Immunology. 7, 48.Research output: Contribution to journal › Article
The profile of P2X7 receptor expression and properties in polarized primary mouse microglia cultures
Jackson, M., Wickens, R., Bailey, S. & Mackenzie, A., 2016, In : Journal of Psychopharmacology . 30, Abstract Supplement to volume 8, p. A98Research output: Contribution to journal › Meeting abstract
Thesis
Amyloid peptides and the vascular system : the link between cerebrovascular angiopathy and Alzheimer's disease
Author: Alsheikh Abubaker, A., Dec 2019Supervisor: Eggleston, I. (Supervisor), Mackenzie, A. (Supervisor) & Pula, G. (Supervisor)
Student thesis: Doctoral Thesis › PhD
Molecular mechanisms of YAP signalling in the context of cancer
Author: Xie, B., 13 Feb 2019Supervisor: De Bank, P. (Supervisor), Mackenzie, A. (Supervisor), Ward, S. (Supervisor) & Mrsny, R. (Supervisor)
Student thesis: Doctoral Thesis › PhD
The Development of Biological Therapeutics
Author: Ali-Hassan, S., 31 Dec 2013Supervisor: Mackenzie, A. (Supervisor) & Watts, A. (Supervisor)
Student thesis: Doctoral Thesis › MPhil
The Functional Expression of N-Methyl-D-Aspartate Glutamate-Type Receptors by Megakaryocytes and Platelets
Author: Hobbs, C., 1 May 2010Supervisor: Mackenzie, A. (Supervisor)
Student thesis: Doctoral Thesis › PhD
The role of fast ATP-Gated P2X receptors in inflammation
Author: Moore, S., 1 Dec 2008Supervisor: Mackenzie, A. (Supervisor)
Student thesis: Doctoral Thesis › PhD