Nano Liquid Chromatography coupled to Electrospray quadrupole Time-of-Flight Mass Spectrometer (nanoLC-ESI-QTOF MS)

Facility/equipment: Equipment

  • Location

    3 S 0.11 University of Bath Claverton Down Bath BA2 7AY

    UK United Kingdom

Equipments Details


Used for intact protein analysis, monitoring changes in glycosylation, and determining degradation products of monoclonal antibodies (mAB) and antibody-drug conjugates (ADC). Also has MS/MS capability for peptide mapping of digested proteins. For identification of complex protein digest a Mascot server is utilised (maintained of seperate PC).


NameMake: AGILENT; Model: G6520BA Q-TOF LC/MS
Acquisition date5/10/11


  • Proteomics
  • Mass spectrometry
  • MS
  • Electrospray ionisation
  • Quadrupole Time-of-Flight
  • QTOF
  • LC
  • NanoLC
  • Liquid chromatography
  • High resolution
  • Accurate mass
  • Protein analysis

Research Output

A rapid and quantitative technique for assessing IgG monomeric purity, calibrated with the NISTmAb reference material

Reader, P., Olkhov, R., Reeksting, S., Lubben, A. T., Hyde, C. & Shaw, A., 1 Sep 2019, In : Analytical and Bioanalytical Chemistry. 411, 24, p. 6487-6496 10 p.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)