Nano Liquid Chromatography coupled to Electrospray quadrupole Time-of-Flight Mass Spectrometer (nanoLC-ESI-QTOF MS)

Facility/equipment: Equipment

  • Location

    3 S 0.11 University of Bath Claverton Down Bath BA2 7AY

    UK United Kingdom

Description

Used for intact protein analysis, monitoring changes in glycosylation, and determining degradation products of monoclonal antibodies (mAB) and antibody-drug conjugates (ADC). Also has MS/MS capability for peptide mapping of digested proteins. For identification of complex protein digest a Mascot server is utilised (maintained of seperate PC).

Details

NameMake: AGILENT; Model: G6520BA Q-TOF LC/MS
Acquisition date5/10/11

Keywords

  • Proteomics
  • Mass spectrometry
  • MS
  • ESI-QTOF MS
  • Electrospray ionisation
  • Quadrupole Time-of-Flight
  • QTOF
  • LC
  • NanoLC
  • Liquid chromatography
  • High resolution
  • Accurate mass
  • MASCOT
  • Protein analysis