Dataset for "Fluorescent Styrene Maleic Acid Copolymers to Facilitate Membrane Protein Studies in Lipid Nanodiscs"



The purpose for obtaining this data was to characterise fluorescent styrene maleic acid copolymers synthesised via RAFT copolymerisation with styrene-analogous fluorophores. Copolymer behaviours were minimally affected and were able to spontaneously form lipid nanodiscs (SMALPs) upon incubation with DMPC vesicles. The potential usefulness of these novel copolymers to membrane protein (MP) research was also assessed. FRET was found to occur between the copolymer annulus and an encapsulated model MP, gramicidin, indicative of proximity. Further, aggregation caused quenching phenomena were used to monitor the transition from aggregated copolymer to nanodisc annulus during SMALP self assembly. Here, both the raw and processed data used in the publication are presented as .xls, .txt, and .pdf files, as indexed in the dataset.
Date made available17 Mar 2022
PublisherUniversity of Bath

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