Dataset for 'Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage'

Formation of crystalline biofilms following infection by Proteus mirabilis can lead to encrustation and blockage of long-term indwelling catheters, with serious clinical consequences. We describe a simple sensor, placed within the catheter drainage bag, to alert of impending blockage via a urinary colour change.

The dataset presented shows data from the characterisation and testing of the prototype sensors, Including: investigation into the fluorescent properties of 5(6)-carboxyfluorescein (CF) (fluorescence output vs CF concentration and fluorescence output vs solution pH), change in pH vs time for P. mirabilis subcultures and bacterial supernatants, corresponding dye release vs time, as well as data from the testing of the sensors within the in vitro bladder model system (change in fluorescence output within the drainage bag and approximate CF concentration with time). Investigation into the initial dye release kinetics was also undertaken and the data presented in this dataset. Standard curves at three different pHs (6,7 and 8) are presented as well as the overlaid release profiles over time.