Dataset for "Development of a mixed-species biofilm model and its virulence implications in device related infections"

  • Naing Tun Thet (Creator)
  • Laura Wallace (Researcher)
  • Toby Jenkins (Project Leader)
  • Anne Wibaux (Researcher)
  • Nick Boote (Researcher)

Dataset

Description

This dataset contains the data underlying the figures presented in "Development of a mixed-species biofilm model and its virulence implications in device related infections". The associated paper reports the development of a simple mixed-species biofilm model using strains of two clinically significant bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, grown on nano-porous polycarbonate membranes on nutrient agar support. The following data tables are included:

- Total number of viable cells (in colony forming units/biofilm) recovered from 8 selected single-species biofilms (Figure 3a).
- Total number of viable cells (in colony forming units/biofilm) recovered from 5 mixed-species biofilms (Figure 3b).
- Fluorescent response of lipid vesicles after incubation with planktonic culture of 20 S. aureus in tryptic soy broth and 10 P. aeruginosa strains in Luria broth (18 hours culture) for 24 hours (Figure 4a).
- Fluorescent response of prototype diagnostic dressing to single-species biofilms after incubation at 33°C for 24 hours (Figure 4b).
- Fluorescent response of prototype diagnostic dressing in triplicate to each mixed-species biofilm, based on positive control dressing with 250 µM 5,6-carboxyfluorescein (Figure 5a).
- Time dependent variation of biofilm cells (colony forming units) and in situ fluorescent response of prototype diagnostic dressing throughout the biofilm formation of S. aureus and P. aeruginosa in mixed-species biofilms (Figure 6a).
- Reduction of viable biofilm cells (colony forming units) in single-species biofilms of S. aureus and P. aeruginosa using 2% octenidine hydrochloride containing hydrogel (Figure 6b).
Date made available8 Mar 2018
PublisherUniversity of Bath
Date of data production1 Jul 2016 - 1 Feb 2017

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