This dataset contains information from a study characterising the Bristol Erythroid Line-A (BEL-A) cell line for mass production, including growth and metabolic kinetics of BEL-A cells, effect of doxycycline, impact of (and tolerance to) lactate and ammonia, and impact of high density culture. Data is provided in an Excel file with individual tabs containing the dataset for each experiment, and comprises live and dead cell numbers, nutrient/metabolite concentrations, and pH measurements.