This dataset provides all the raw data collected for a trial investigating the impact of long-term physical inactivity in the form of head-down bed rest on adipose tissue immunometabolism in young (20–45 yrs), healthy males. This project was conducted as part of a larger, international investigation conducted by the European Space Agency (AO-BR-13) in the MEDES Facility, Toulouse, France.
Participants were recruited by international advertisement. The trial was conducted in accordance with Guidelines for Conducting Bed Rest Studies (Heer et al, 2009). All participants were confined to the clinical facility for 14 days prior to commencing bed rest, which was then undertaken for 60 days, followed by a 14 day recovery period. Baseline Characteristics data at the moment of entry to the clinical facility is reported in Tab 1. The ESA medical staff at the MEDES facility undertook the day-to-day running of the study. Diet was formulated and produced in-house. Exact portion sizes and foods/fluids not consumed were recorded by weighed inventory, along with food types consumed at each meal on each day of the study. Diet data (macro and micronutrient) for days pertaining to CGMS analyses conducted here (BDC-8, -7 and HDT+53, +55) are reported in Tab 2. Bloods were taken on the mornings of the adipose biopsies, immediately upon awaking, in the fasted state, data are presented in Tab 8 for plasma protein analysis, and Tab 3 for PBMC analysis by flow cytometry. Following blood extraction, participants underwent and adipose tissue biopsy conducted by a surgeon. Adipose was extracted by needle aspiration from the abdominal subcutaneous adipose tissue, 5cm lateral to the umbilicus. Whole adipose tissue was partitioned as outlined in Figure B, below. Immunoblotting (Tab 10) and rtPCPR (Tab 9) was carried out on whole- adipose tissue; ex vivo culturing of whole adipose tissue (Tab 7) was performed for 3 h upon collection; flow cytometry was also conducted on digested adipose tissue (Tab 3). For 5 days pre- and at the end of bed rest continuous glucose monitoring probes were inserted into the back of the participants arm, with the probe inserted subcutaneously. These data are presented in Tab 5. Urine samples (2mL) were collected from each void across a 24 hour period before and at the end of bed rest and analysed for glucose concentrations (Tab 6. All biological data can be found in respective tabs.
Heer, M., Liphardt, A., and Frings-Meuthen, P. (2009). Standardisation of bed rest study conditions. Hamburg: DLR Institute of Aerospace Medicine.